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园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1095-1097.

• 研究报告 • 上一篇    下一篇

黄瓜抗白粉病基因AFLP标记的SCAR转换

杜胜利;张桂华;李淑菊;王 鸣   

  1. (1 天津科润黄瓜研究所, 天津300192; 2 西北农林科技大学园艺学院, 杨凌712100; 3 天津市农业生物技术研究中心, 天津300192)
  • 收稿日期:2005-01-11 修回日期:2005-04-04 出版日期:2005-12-25 发布日期:2005-12-25

Development of a SCAR Marker Linked to Cucumber Powdery Mildew Resistance Gene from an AFLP Marker

Du Shengli;Zhang Guihua;Li Shuju;Wang Ming   

  1. (1 Tianjin Kerun Cucumber Research Institute, Tianjin 300192, China; 2Department of Horticulture, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China; 3 Tianjin Agricultural Biotechnology Research Center,Tianjin 300192, China)
  • Received:2005-01-11 Revised:2005-04-04 Online:2005-12-25 Published:2005-12-25

摘要: 将黄瓜抗白粉病相关基因的1个共显性AFLP标记转换为简单实用的SCAR 标记。应用该SCAR标记对36个已知田间白粉病抗性的黄瓜材料进行了盲测, 测定结果与其田间抗病性吻合率高达
94.5% , 表明该标记可以用于黄瓜白粉病的分子鉴定。

关键词: 黄瓜, 白粉病, 抗性相关基因, 分子标记, AFLP, SCAR

Abstract: The co-dominant AFLP marker was successfully converted into co-dominant SCAR marker.Powdery mildew resistance of 36 known cucumber lines were tested by SCAR markers, and the results indicated that the resistance tested was consistent with their field performance by 94.5% , which further confirmed
the feasibility of the SCAR marker in MAS for cucumber powdery mildew.

Key words: Cucumber, Powdery mildew, Resistance-related gene, Molecular marker, AFLP, SCAR

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