https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1084-1087.

• 研究报告 • 上一篇    下一篇

‘雪青’梨叶片高频再生体系的建立

汤绍虎;孙 敏;周启贵;龙 云;李道高
  

  1. (1 西南农业大学园艺园林学院, 重庆400716; 2 西南师范大学生命科学学院, 重庆400715)
  • 收稿日期:2005-01-04 修回日期:2005-03-18 出版日期:2005-12-25 发布日期:2005-12-25

Establishment of High Frequently Regeneration System from Leaves of‘Xueqing’Pear

Tang Shaohu;Sun Min;Zhou Qigui;Long Yun;Li Daogao

  

  1. ( 1College of Horticulture and Gardening, Southwest Agricultural University, Chongqing 400716, China; 2College of Life Science, Southwest Normal University, Chongqing 400715, China)
  • Received:2005-01-04 Revised:2005-03-18 Online:2005-12-25 Published:2005-12-25

摘要: 以‘雪青’梨叶片为外植体, MS为基本培养基, 培养温度(25 ±1) ℃, 光照强度2 000 lx,
14 h /d, 继代周期30 d。在MS + 2,4-D 2 mg·L - 1 + 6-BA 0.5 mg·L - 1培养基中, 外植体脱分化率达100% ,愈伤组织增殖倍数达12.05; 在MS + 6-BA 5 mg·L - 1 + IAA 0.1 mg·L - 1中, 不定梢诱导率达100% , 在MS+ 6-BA 2 mg·L - 1 + IAA 0.1 mg·L - 1 +CH 100 mg·L - 1中, 1~6代不定梢平均繁殖系数达7; 在1 /2MS +IBA 2 mg·L - 1 + 6-BA 0.5 mg·L - 1 +AC 500 mg·L - 1中, 不定梢生根率达67.50%; 68株试管苗移栽到珍珠岩营养钵中, 30 d成活46株, 成活率为67.65 %。

关键词: 梨, 组织培养, 植株再生

Abstract: Leaves excised from one-year old shoots of‘Xueqing’pear ( Pyrus sp. ) were used asmaterials to induce adventitious shoots on MS medium supplied with different combinations of p lant growth regulators, 8 g·L-1 agar and 30 g·L-1 sucrose, pH = 5.8 in a growth chamber set at a 14 h photoperiod, a 2 000
lx photosynthetic density and a (25 ±1) ℃ temperature. 100% of the leaf explants dedifferentiated onMS + 2,4-D 2 mg·L - 1 + 6-BA 0.5 mg·L-1 and calli increased 12.05 times in a 30-day subculture period. All the calli could regenerate adventitious shoots after transferred on MS + 6-BA 5 mg·L-1 + IAA 0.1 mg·L - 1 and the shoots proliferated about 7 times in every subculture during one to six subcultures on MS + 6-BA 2 mg·L - 1 + IAA 0.1 mg·L - 1 +CH 100 mg·L - 1. 67.50% of shoots rooted on 1 /2MS + IBA 2 mg·L - 1 + 6-BA 0.5 mg·L - 1 +AC 500 mg·L - 1. Of 68 plantlets, 46 plantlets (67.65% ) were acclimatized after transplanting to matrix pearlite for 30 days.

Key words: Pear, Tissue culture, Plant regeneration

中图分类号: