https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1030-1033.

• 研究论文 • 上一篇    下一篇

人乳铁蛋白基因在番茄中的优化表达

曹慧颖;郭三堆   

  1. (中国农业科学院生物技术研究所, 北京100081)
  • 收稿日期:2005-03-04 修回日期:2005-04-22 出版日期:2005-12-25 发布日期:2005-12-25

Expression of Human Lactoferr in Gene in Transgenic Tomato Plants

Cao Huiying;Guo Sandui   

  1. (Biotechnology Research Institute, the Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2005-03-04 Revised:2005-04-22 Online:2005-12-25 Published:2005-12-25

摘要: 采用农杆菌介导法, 将表达载体pB I121LF中的人乳铁蛋白(LF) 基因(根据植物偏好密码子人工合成) 导入番茄, 经卡那霉素筛选, 获得了再生植株。经PCR和Southern鉴定, 证明部分番茄基因组中已经整合了lf基因。Western检测表明, 基因在果实中得到了表达。

关键词: 乳铁蛋白, 转基因, 番茄, 生物反应器

Abstract: The plant expression vector pBI121LF contained artificial synthesized human lactoferrin gene was constructed and introduced into tomato by Agrobacterium -mediated. Twenty-three regenerated plants were obtained through kanamycin-resistant selection. Itwas demonstrated that lf gene had been integrated into tomato genome by PCR and Southern blot analysis. Western blot analysis showed that human lactoferrin had expressed in transgenic tomato fruits. Itmade the basic research for transgenic tomato as healthy food.

Key words: Lactoferrin, Transgenic, Tomato, Bioreactor

中图分类号: