黄花菜受精过程的研究

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1013-1020.

黄花菜受精过程的研究

申家恒;申　业;王艳杰;袁秋红;于春光

(¹ 哈尔滨师范大学生命与环境科学学院生物系, 哈尔滨150080; ²黑龙江大学生命科学学院生物工程系, 哈尔滨150080)

收稿日期:2005-01-17 修回日期:2005-05-23 出版日期:2005-12-25 发布日期:2005-12-25

Studies on Fertilization of Hemerocallis citrina Baroni

Shen Jiaheng;Shen Ye;Wang Yanjie;Yuan Qiuhong;Yu Chunguang

( ¹Department of Biology, College of Life and Environment Science, Harbin Normal University, Harbin 150080, China;²Department of Biological Engineering, College of Life Science, Heilongjiang University, Harbin 150080, China)

Received:2005-01-17 Revised:2005-05-23 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 利用常规石蜡切片技术对黄花菜受精过程进行了详细的研究, 获得如下结果: 1. 授粉后1～2 h花粉在柱头上萌发长出花粉管, 只有生殖细胞进入花粉管内。2. 花粉管在花柱道内生长历经20～22 h,生殖细胞分裂形成2个精子。3. 花粉管在子房室中轴胎座表面生长经历2～4 h。4. 授粉后26～28 h花粉管进入1个助细胞, 释放2个精子。5. 精卵融合经历4～6 h, 形成合子。6. 精核与次生核融合经历2～4 h, 形成初生胚乳核。7. 自花粉在柱头萌发至合子形成, 受精过程持续总时间为29～36 h。8. 合子分裂发生在授粉后39～48 h。9. 讨论了研究结果对黄花菜遗传育种与分子育种的意义。

关键词: 黄花菜, 双受精, 花粉管通道法

Abstract: Observed the process of fertilization in Hemerocallis citrina Baroni in detail by means of paraffin wax. The main results are as follows: 1. After artificial pollination 1 - 2 hours, pollens germinated at the top of stigma, growing out pollen tube, where only generative cell can get into. 2. Pollen tube prolongs in
stylar canal for 20 - 22 hours, in concomitant with generative cell dividing into two sperms. 3. Pollen tube prolongs on the surface of axile placenta in locule for 2 - 4 hours. 4. After artificial pollination 26 - 28 hours, pollen tube get into one of synergids, and releases two sperms. 5. It takes 4 - 6 hours to egg-sperm fusion, and then bring out zygocyte. 6. The formation of primary endosperm nucleus results from 2 - 4 hours’egg-secondary nucleus fusion. 7. The duration of the whole fertilization is 29 - 36 hours from pollen germination to the formation of zygocyte. 8. After artificial pollination 39 - 48 hours, zygocyte begins to divide. 9. Discussed the significance of the result of study to genetic breeding and molecular breeding.

Key words: Hemerocallis citrina Baroni, Double fertilization, Pollen-tube pathway

中图分类号:

S 644.3

申家恒;申　业;王艳杰;袁秋红;于春光. 黄花菜受精过程的研究[J]. 园艺学报, 2005, 32(6): 1013-1020.

Shen Jiaheng;Shen Ye;Wang Yanjie;Yuan Qiuhong;Yu Chunguang. Studies on Fertilization of Hemerocallis citrina Baroni[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1013-1020.

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