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园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 127-130.

• 研究报告 • 上一篇    下一篇

萝卜RsCYP86MF 基因cDNA全序列克隆及结构特征分析

王玲平;曹家树;叶纨芝;向珣;余小林   

  1. (浙江大学蔬菜研究所, 杭州310029)
  • 收稿日期:2004-02-23 修回日期:2004-05-10 出版日期:2005-02-25 发布日期:2005-02-25

Cloning and Characterization of Full-length cDNA of RsCYP86M F Genefrom Raphanus sativus L.

Wang Lingping;Cao Jiashu;Ye Wanzhi;Xiang Xun;Yu Xiaolin   

  1. ( Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, China)
  • Received:2004-02-23 Revised:2004-05-10 Online:2005-02-25 Published:2005-02-25

摘要: 本研究通过设计保守的基因特异引物, 运用SMART RACE RT - PCR技术从‘圆白’萝卜中
获得一个细胞色素P450基因, 命名为RsCYP86M F, 其cDNA全长1818 bp, 含有1581 bp的完整开放阅读框, 编码526个氨基酸, 该基因编码的蛋白序列与白菜的细胞色素P450基因编码的氨基酸序列同源性高达90% , 且有很高的功能区段保守性; 对其可能编码的蛋白质二级结构进行预测发现, 该蛋白质具有细胞色素P450典型的保守结构域FNAGPRLCIG, 及N - 糖基化位点等结构域。

关键词: 萝卜, RsCYP86MF基因, cDNA, 克隆, 分析

Abstract: By SMART RACE RT-PCR strategywith gene specific p rimers, the full-length cDNA of a cytochrome P450 gene named RsCYP86M F was cloned from Raphanus sativus L. The cDNA was 1818 bp, it encompassed a open reading frame (ORF) with 1581 bp encoding 526 amino acid residues. the sequences of deduced amino acids encoded by cytochrome P450 genes showed the high identity of 90% between Raphanus sativus L. and Brassica campestris subsp. chinensis, and also highly conserved domains of function. It had a conserved domain of cytochrome P450 cysteine heme-iron ligand signature ( FNAGPRLCIG) , which is typical of cytochrome P450, and the structure domains such as N-glycosylation.

Key words: Raphanus sativus L., RsCYP86M F gene, cDNA, Cloning, Characterization

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