关键词: 葡萄, 根系分泌物, 化感作用, 化感物质

Abstract:

In order to explore the allelopathy of grape root exudates，tissue cultured plantlets of Red Globe（Vitis vinifera L.）in laboratory and cutting seedlings of Beta（V. riparia × V. labrusca）by pot experiment were used to examine the effects of grape exudates on plant growth. Laboratory tests showed 0.01，0.10，0.50 g · mL-1（root dry weight/root exudates volume）grape root exudates significantly inhibited growth of grape plantlets，the inhibition being stronger at higher root exudate concentrations. Pot experiment indicated that low-concentration root exudates promoted plant growth，and increased soluble sugar content，starch content and protein content of leaves. The difference of PAL and SOD activities betweenthe treatments and the control were not significant，and MDA content decreased under low concentration. High-concentration root exudates inhibited plant growth significantly，and PAL and SOD activity were higher than the control by 69.16% and 10.35% respectively，and MDA content increased. p-Hydroxybenzoic acid and salicylic acid were determined by LC-MS in grape root exudates，which had significant allelopathy effect on tissue culture plantlets. Other compounds in root exudates of grape were inferred as palmitic acid，1-heneicosanol，citric acid，aconitic acid，β-sitosterol，phthalic acid and gallic acid.

Key words: grape, root exudates, allelopathy, allelochemicals