应用cDNA-AFLP技术分离草菇冷诱导表达基因

园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 54-59.

应用cDNA-AFLP技术分离草菇冷诱导表达基因

郭丽琼;林俊芳;杨丽卿;刘瑞瑞

(¹华南农业大学食品学院生物工程系, 广州 510640; ² 中国热带农业科学院热带作物生物技术国家重点实验室, 海口 571101)

收稿日期:2004-04-21 修回日期:2004-06-17 出版日期:2005-02-25 发布日期:2005-02-25

Isolation of Cold Stress Genes through cDNA-AFLP from Volvariella volvacea

Guo Liqiong;Lin Junfang;Yang Liqing;Liu Ruirui

(¹Department of Bioengineering, College of Food Sciences, South China Agricultural University, Guangzhou 510640, China; ² State Key Laboratory of Tropical Crop Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)

Received:2004-04-21 Revised:2004-06-17 Online:2005-02-25 Published:2005-02-25

摘要/Abstract

摘要： 采用cDNA-AFLP技术筛选获得了5个草菇冷诱导表达基因片段VC1、VC2、VC3、VC4 和
VC5。测序结果表明, 5个DNA片段大小分别为247 bp、219 bp、172 bp、350 bp和171 bp。同源性BLAST搜索结果显示, VC1、VC2、VC3、VC4和VC5片段目前都没有同源的核酸序列; VC1片段翻译的蛋白质序列与粗糙脉孢菌(Neurospora crassa) 的假拟蛋白具有一定的同源性, VC2片段翻译的蛋白质序列与水稻的AMP脱氨酶具有一定的同源性, VC3片段翻译的蛋白质序列与Danio rerio的糖原合酶激酶3α具有较高的同源性, VC4片段翻译的蛋白质序列与Leuconostocm esenteroides的假拟蛋白有一定的同源性, VC5片段翻译的蛋白质序列没有搜索到同源序列。

关键词: 草菇, cDNA-AFLP, 冷诱导表达基因

Abstract: Five cold stress gene fragments were isolated through cDNA-AFLP from Volvariella volvacea and were designated as VC1, VC2, VC3, VC4 and VC5 respectively. DNA sequence results indicated that these five DNA fragments had 247 bp, 219 bp, 172 bp, 350 bp and 171 bp respectively. BLAST search results showed that VC1, VC2, VC3, VC4 and VC5 had no significant homologous nucleotide sequences found in databases. Postulated p rotein sequences of VC1, VC2 were homologous with the hypothetical protein of
Neurospora crassa and the putative AMP deaminase of Oryza sativa, espectively. Postulated protein sequences of VC3, VC4 were homologouswith the glycogen synthase kinase three alpha in Danio rerio and the hypothetical protein in Leuconotocm esenteroides respectively. But postulated protein sequence of VC5 had not found the significant homologous p rotein sequence in the databases yet.

Key words: Volvariella volvacea, cDNA-AFLP, Cold stress gene

中图分类号:

S646.1
Q812

郭丽琼;林俊芳;杨丽卿;刘瑞瑞. 应用cDNA-AFLP技术分离草菇冷诱导表达基因[J]. 园艺学报, 2005, 32(1): 54-59.

Guo Liqiong;Lin Junfang;Yang Liqing;Liu Ruirui. Isolation of Cold Stress Genes through cDNA-AFLP from Volvariella volvacea[J]. ACTA HORTICULTURAE SINICA, 2005, 32(1): 54-59.

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