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园艺学报 ›› 2003, Vol. 30 ›› Issue (4): 404-408.

• 研究论文 • 上一篇    下一篇

转反义LeETR1基因番茄采后生理特性的研究

杨虎清;应铁进;向庆宁;杜荣茂
  

  1. ( 浙江大学食品科学与营养系,杭州3113029; 浙江大学园艺系,杭州311029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-25 发布日期:2003-08-25

Characters of Postharvest Physiology of Antisense LeETR1 Transgenic TomatoFruits

Yang Huqing ,Ying Tiejin ,Xiang Qingning2,and Du Rongmao   

  1. ( Department ofFood Science and Nutrition,Zhejiang Unirersity,Hom~hou 310029,University,Hangzhou 310029, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

摘要: 转反义LeETR1乙烯受体基因番茄的采后生理性状与普通番茄不同。转基因番茄的呼吸作用
受到抑制,呼吸高峰只有对照的52%,乙烯释放高峰的出现比对照果实推迟10 d。转基因番茄的叶绿素降解和番茄红素的合成受到显著抑制,果实不能形成正常的红色。在成熟过程中,转基因番茄的纤维素酶、多聚半乳糖醛酸酶活性显著低于对照,延缓了果肉的软化。推测LeETR1和番茄的成熟有着密切的关系。

关键词: 番茄, 转基因, 乙烯, 成熟, 色素

Abstract: In this paper we studied the post—harvest physiology of antisense LeETR 1 transgenic tomato fruits. Respiratory rate of transgenic fruits was significantly lower than contro1. Ethylene production peak of transgenic fruits appeared 10 d after contro1. Chlorophyll degration an d lycopene biosynthesis Was inhibited in transgenic fruits.After 30 days in normal room,transgenic tomatos eventually developed an orange colour but never turned red.During the course of ripening,cellulase and pely~ acturonase activity of transgenie fruits wel'B signitieantly lower than control and the firmness of transgenic fruits declined more slowly.These datas suggest that LeETR1 Was closely correlated with the ripe ning of tomato fruits.

Key words: Tomato, Transgenic, Ethylene, Ripening, Pignent

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