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园艺学报 ›› 2003, Vol. 30 ›› Issue (4): 399-403.

• 研究论文 • 上一篇    下一篇

荔枝品种亲缘关系的AFLP分析

易干军;霍合强;陈大成;黄自然;蔡长河;邱燕萍
  

  1. ( 广东省农业科学院果树研究所,广州510640; 华南农业大学,广州510640)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-25 发布日期:2003-08-25

Studies on Genetic Relationship among Litchi Varieties by Usmg AFLP

Yi Ganjun,Huo Heqiang,Chen Dacheng,Huang Ziran,Cai Changhd,and Qiu Yanping
  

  1. ( Institute of Fndt Tree Research, Guangdong Academy of Agricultural & nce, Giizilg-zhott 510640,China; South China Agriclural University,Gtlzlllg'zhou 510640,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

摘要: 应用AFLP技术,对39个荔枝品种进行了遗传多样性分析及分类研究。筛选出适于荔枝AFLP
分析的最佳引物组合3对,分别为EcoRI AAC+MseI CTG,EcoPd AGC+MseI CAT,EcoRI ACC+Msel CAT。在分子水平上,荔枝品种的遗传多样性并非形态学性状所体现的那样丰富。AFLP分析将39个荔枝品种分成了6组,与传统的以果皮龟裂片隆起类型为分类标准的分类没有一致性。初步建立起荔枝主要栽培品种的分子标记标准图谱。应用AFLP技术对来自两个不同地方的两个荔枝品种(桂味、妃子笑)进行了鉴别。

关键词: 荔枝, 亲缘关系, AFLP

Abstract: Identification and classificati0n of 39 litchi varieties were studied based on A兀P analysis.Threepair of primers(EcoRI AAC+MseI CTG,EcoRI AGC+MseI CAT,EcoRI ACC+MseI CAT)were screenedfrom 64 pare ofprimers.rI1le an alysis ofgenetic diversity among litchi varieties indicated on morphological level lessabundant than molec~ar leve1.The cluster analysis results showed that the 39 cuhivars could be divided into 6groups.It is diferent from the traditional classification system based on peel morphology including peel smoothness,scale size.scale arrangement.rI1le results showed peel morphology Call not be used for litchi varieties classificationas the only criterion.The molecular marker standard map of the main litchi varieties Was established .Two varieties(Litchi chinensis ‘Guiwei’and Litchi chinensis ‘FeizSxJao’) come from diferent arIea were identified.

Key words: Utchi, A兀P, Genetic relatiouship

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