关键词: 菠萝, 胚性细胞, 体细胞胚, 起源, 组织细胞学

Abstract: Histocytology study was done by paraffin section method on the globular embryogenesis in pineapple（Ananas comosus）. The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division. The cells of proembryo had strong division ability, and there were distinct difference in shape and obvious boundary between them/it and the surrounding tissues. A suspensor-like structure was formed on the bottom of the proembryo. Along with the constant growth of the proembryo, its shape converted from long-ellipse to obovate, at last it evolved as a globular embryo with smooth surface, and there were absciss layer in the joint of the bottom of suspensor and matrix tissue. At the same time, cell differentiation appeared in the globular embryo. Pineapple somatic embryogenesis has two original modes，endogenous origin and exogenous origin. Additionally, there were ubiquitous uneven，desynchronization and secondary embryosphenomenon during somatic embryogenesis in pineapple callus.

Key words: pineapple, Ananas comosus, embryogenic cell, somatic embryos, origin, histocytology