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园艺学报 ›› 2010, Vol. 37 ›› Issue (3): 475-478.

• 研究报告 • 上一篇    下一篇

13个阿月浑子品种的RAPD分析

王 超;郝爱丽;白志英;路丙社   

  1. 1 河北农业大学林学院,河北保定071001;2 河北农业大学园林与旅游学院,河北保定071001;3 河北农业大学生命科学学院,河北保定071001)
  • 收稿日期:2009-12-03 修回日期:2010-01-03 出版日期:2010-03-25 发布日期:2010-03-25

RAPD analysis of 13 Pistachio(Pistacia vera)Cultivars

WANG Chao;HAO Ai-li;BAI Zhi-ying;LU Bing-she   

  1. (1 Forestry College,Hebei Agricultural University,Baoding,Hebei 071001,China;2 Landscape Architecture and Tourism College,Hebei Agricultural University,Baoding,Hebei 071001,China;3 Life Science College,Hebei Agricultural University,Baoding,Hebei 071001,China)
  • Received:2009-12-03 Revised:2010-01-03 Online:2010-03-25 Published:2010-03-25

摘要: 为探明引进的阿月浑子品种间的亲缘关系,采用RAPD 标记技术对引入的12 个国外品种和‘新疆优株’进行了分析。用筛选的21 条10 bp 随机引物进行PCR 扩增可扩增出137 个位点,其中多态位点122 个,多态位点比率89.05%;品种间遗传距离在0. 2015 ~ 0.5163 之间,表明各品种间存在一定的遗传差异。UPGMA 聚类分析表明,13 个阿月浑子品种在遗传距离0.40 处可划分为3 个类群,第1 类包括 ‘Kerman’、‘Larnaka’、‘M-38’、‘M-P3’、‘Ashoury’、‘N’、‘Joley’、‘Aegina’、‘Avidon’、‘B’和‘Peter’11 个品种;第2 类为‘Xinjiang select tree’品种;第3 类为‘Mateur’品种。

关键词: 阿月浑子, 遗传多样性, RAPD 分析

Abstract:

In order to analyze pistachio genetic relationship, random amplified polymorphic DNA (RAPD) was used to study the classification and identification of 13 pistachio (Pistacia vera L.)cultivars which includs 12 exotic varities and one native selects of Xinjiang. Twenty-one 10 bp primers that selected
from eighty arbitrary primers were applied for amplifying the pistachio DNA. Total 137 bands were produced, among which 122 bands (89.05%) were polymorphic. Genetic distances among the cultivars were estimated based on the amount of band sharing and ranged from 0.2015–0.5163, it is indicated that there are genetic differences among the cultivars. UPGMA cluster analysis showed that 13 pistachio varieties can be divided into three groups in the genetic distance of 0.40 level: The first category includes eleven varieties, the second category is the ‘Xinjiang selection’ and the third category is ‘Mateur’ varieties.

Key words: Pistacia vera L., genetic diversity, RAPD

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