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园艺学报 ›› 2010, Vol. 37 ›› Issue (3): 428-434.

• 观赏植物 • 上一篇    下一篇

ABA对‘西伯利亚’百合试管鳞茎发育及休眠的影响

赵海涛;刘 春;明 军;穆 鼎   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 收稿日期:2009-12-23 修回日期:2010-02-03 出版日期:2010-03-25 发布日期:2010-03-25

Effects of ABA on the Development and Dormancy in the Bulblets of Oriental Hybrids Lily ‘Siberia’ Generated in Vitro

ZHAO Hai-tao;LIU Chun; MING Jun;MU Ding   

  1. (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China)
  • Received:2009-12-23 Revised:2010-02-03 Online:2010-03-25 Published:2010-03-25

摘要: 以东方百合杂种系的‘西伯利亚’百合(Lillium ‘Siberia’)为材料,研究了ABA 及其合成抑制剂氟啶酮(Fluridone)对试管鳞茎发育及休眠的影响,结果表明:在黑暗条件下生长原基只发育成鳞片,加入ABA 合成抑制剂Fluridone 后鳞片和鳞片叶同时生成,在含有Fluridone 的培养基中加入外源ABA 后,Fluridone 促进鳞片叶片生成的作用被逆转,说明外源ABA 可以抑制鳞片叶的生成。Fluridone可以降低鳞茎的休眠程度,当外源ABA 与其同时存在时,Fluridone 的这种作用会被逆转,说明外源ABA可以促进鳞茎休眠。鳞茎的休眠程度在15、20 和25 ℃培养条件下会随温度升高而加深,在25 ℃条件下鳞茎进入完全休眠,低浓度的外源ABA 对于15、20 和25 ℃条件下鳞茎的休眠没有促进作用,说明温度对休眠的影响可能不是通过ABA 发挥作用的,除了ABA 外还存在尚不清楚的物质影响着休眠形成。百合试管鳞茎休眠深浅与其形态发育没有直接的因果关系。

关键词: 百合, 脱落酸, 氟啶酮, 试管鳞茎, 休眠

Abstract:

The effects of abscisic acid (ABA) on the development of morphology and dormancy of bulblets in vitro of Oriental hybrids‘Siberia’were investigated. The results showed that the primordium was developed into scales only in the dark. While Fluridone, an inhibitor of ABA-synthesis, was applied during in vitro culture, the primordium was developed into scales or leaves. While exogenous ABA applied simultaneously with Fluridone, the effect of Fluridone was nullified. It indicated that the exogenous ABA can inhibite the formation of scal-leaves while endogenous ABA-synthesis was inhibited. Fluridone can reduce the levels of dormancy, and the function can be reversed while adding exogenous ABA. It
suggested that the exogenous ABA can promote the development of dormancy. The dormancy levels of in vitro bulblets cultured at 15, 20 and 25 ℃ will be raised , but the low concentration exogenous ABA had no effect on the bulblets at 15, 20 and 25 ℃ with a low level of dormancy. It speculates that the effect of temperature on the dormancy maybe not play by ABA, an unknown factor , in addition to ABA, still played a key role in dormancy development. There is no relation between the morphology development
and the dormancy levels.

Key words: lily, abscisic acid, fluridone, bulblets in vitro, dormancy development

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