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园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 121-128.

• 研究报告 • 上一篇    下一篇

苹果属观赏海棠实生单株亲本的AFLP鉴定

赵天田;沈红香;姚允聪3;曹庆芹;宋婷婷   

  1. (北京农学院植物科学技术学院, 北京102206)
  • 收稿日期:2009-05-05 修回日期:2009-11-16 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 姚允聪

Identification of Parentage of Ornamental Crabapple Seedlings Using AFLPMarkers

ZHAO Tian-tian;SHEN Hong-xiang; YAO Yun-cong*;CAO Qing-qin;SONG Ting-ting   

  1. ( Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China)
  • Received:2009-05-05 Revised:2009-11-16 Online:2010-01-25 Published:2010-01-25
  • Contact: YAO Yun-cong

摘要: 应用AFLP技术, 对以苹果属观赏海棠品种王族(Malus ‘Royalty’) 为母本, 以5个材料为父本的38个实生单株进行多态性分析。结果表明: 筛选出的9对分辨率高的引物共扩增出349条带, 其中多态性带303条, 多态位点百分率达86.8%。根据聚类分析和电泳图谱扩增得到亲本材料的特征带, 鉴定出19株实生单株的父本可能为‘宝石’ (Malus‘Jewelberry’) ; 7株实生单株的父本可能为海棠花(Malus spectabilis Borkh. ) ; 3株实生单株的父本可能为草莓果冻(Malus‘Strawberry Parfait’) ; 不能确定其父本的实生单株有9株。

关键词: 苹果属, 观赏海棠, 实生单株, AFLP, 亲本鉴定

Abstract: The polymorphism was analyzed by AFLP in 38 Ornamental Crabapple seedlings from female parent, Malus‘Royalty’, and 5 possible male parents. Results showed that the total 349 informative AFLP fragments were generated using the selected 9 EcoR Ⅰ - Mse Ⅰ p rimer combinations with high resolution capacity. Among them there were 303 polymorphic fragments with a polymorphism percentage of 86.8%. According to the characteristic bands of electrophoretogram and Cluster Analysis, the male parents of 19, 7 and 3 seedlings were identified to be Malus‘Jewelberry’, Malus spectabilis Borkh. and Malus‘Strawberry Parfait’respectively. The remnant 9 seedlings could not be confirmed or identified.

Key words: Malus, Ornamental Crabapple, seedling, AFLP, identification of parentage

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