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园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 39-46.

• 蔬菜 • 上一篇    下一篇

利用高代回交群体定位野生醋栗番茄发芽期耐盐QTL

潘颖;王孝宣;杜永臣;胡鸿;高建昌;国艳梅;戴善书;朱德蔚   

  1. (中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2009-08-26 修回日期:2009-11-11 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 杜永臣;胡鸿

Mapping QTLs Conferring Salt Tolerance During Germination in Solanum pimpinellifolium Using AB-QTL

PAN Ying;WANG Xiao-xuan;DU Yong-chen;HU Hong;GAO Jian-chang;GUO Yan-mei;DAI Shan-shu;ZHU De-wei   

  1. ( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2009-08-26 Revised:2009-11-11 Online:2010-01-25 Published:2010-01-25
  • Contact: DU Yong-chen;HU Hong

摘要: 以野生醋栗番茄( Solanum pimpinellifolium ) LA722和栽培番茄( Solanum lycopersicum ) 9706为材料, 结合发芽期大规模盐胁迫筛选方法和AB-QTL 技术进行耐盐QTL 定位和转育研究, 建立了包括280个BC3 S1单株的高代回交群体, 利用26个CAPS、66个SSR和39个SRAP多态性标记构建了分子遗传图谱并进行了耐盐性QTL定位。利用发芽20、24、28 d的发芽指数分别检测耐盐相关的QTL, 确定发芽24 d时的指数为检测QTL的最优指标, 共检测出11个与耐盐相关的QTL, 8个来自于野生醋栗番茄LA722。研究获得了58个极耐盐株系, 对部分耐盐株系进行QTL qST-8-1 和qST-9-2 的基因型分析, 其中230-1和230-3株系含QTL qST-8-1, 228-4株系含主效QTL qST-9-2, 231-6, 210-7, 226-1, 226-6, 226-7和204-7株系同时含有2个主效QTL, 这些株系的农艺性状接近栽培番茄9706, 可作为耐盐育种种质。

关键词: 番茄, 耐盐, 种子萌发, AB-QTL

Abstract: An advanced back-cross BC3 S1 population consisted of 280 individuals was constructed from the cross between Solanum lycopersicum 9706 and Solanum pimpinellifolium LA722 after large scale screening under salt stress during seed germination of each backcross family. One hundred and thirty one polymorphic markers including 26 CAPS, 66 SSR and 39 SRAP were used to construct a linkage map. Using germination index of 20, 24 and 28 d to detected QTLs respectively, and it confirmed that germination index of 24 d is the optimum index. A total of 11 favourable QTLs of salt tolerance were detected using MQM mapping methods, of which 8 QTLs were derived from salt-tolerant wild parents LA722. All significantQTL s accounted for 73.2% of the totlal phenotypic variation. Fifty eight families with high salt tolerance and favorable economic traits were obtained. Family 230-1 and 230-3 contained qST-8-1 locus, family 228-4 contained qST-9-2 locus, while 231-6, 210-7, 226-1, 226-6, 226-7, 204-7 contained both QTLs. These families had similar agronomic traits with 9706 and could be used as breeding materials.

Key words: tomato, salt tolerance, seed germination, AB-QTL

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