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园艺学报 ›› 2010, Vol. 37 ›› Issue (2): 263-276.

• 观赏植物 • 上一篇    下一篇

新铁炮百合单倍体植株的诱导

韩秀丽;田晓明;贾桂霞*   

  1. (北京林业大学园林学院,国家花卉工程技术研究中心,北京100083)
  • 收稿日期:2009-03-26 修回日期:2010-01-21 出版日期:2010-02-25 发布日期:2010-02-25

Induction of Haploid Plantlets for Lilium × formolongi

HAN Xiu-li;TIAN Xiao-ming;JIA Gui-xia*   

  1. (College of Landscape Architecture, Beijing National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing 100083, China)
  • Received:2009-03-26 Revised:2010-01-21 Online:2010-02-25 Published:2010-02-25

摘要: 在新铁炮百合‘雷山1 号’减数分裂和小孢子发育进程研究的基础上,确定了与小孢子不同发育时期相对应的花蕾长度和花药颜色等外部形态特征;筛选出‘雷山1 号’诱导愈伤组织适宜的培养基: 基本培养基为MS 改良培养基,添加2,4-D 0.5 mg · L-1、KT 4.0 mg · L-1,蔗糖浓度30 ~ 90 g · L-1。对不同发育阶段花药诱导培养的结果显示:最佳诱导时期为小孢子发育的早、中期,即花蕾长度为24 ~ 25 mm 时,诱导率达44%。将花药愈伤组织转移至MS 添加2.0 mg · L-1NAA,可诱导分化形成花粉植株,分化率达100%,其中单倍体达43%。

关键词: 新铁炮百合, 小孢子, 单倍体

Abstract: Abstract: Based on the meiosis and microspore development of Lilium × formolongi ‘Raizan 1’, the length of flower bud and the colors of anther relative to the development stages were determined. The suitable media was selected for haploid callus induction of the ‘Raizan 1’, that was improved MS supplemented with 2,4-dichlunruphenoxyacetic(2,4-D)0.5 mg · L-1 and kinetin(KT)4.0 mg · L-1. The results showed the effects of sucrose concentration in the 30–90 g · L-1 were not significant on the microspore callus induction. Microspores at early and mid-stage were more suitable for culture. Induction percentage of haploid calli were 44%, as bud length were 24–25 mm. Plantlet was obtained when haploid calli was transferred to the MS medium supplemented with 2.0 mg · L-1 naphthaleneacctic acid(NAA). The induction rate of plantlets were 100%,there into,plantlets of haploid were 43%.

Key words: Lilium × formolongi, microspore, haploid