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园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1767-1774.

• 蔬菜 • 上一篇    下一篇

新疆哈密瓜永久遗传图谱构建及比较分析

陆芳1, 2 ; 许勇2 ;赵越1 ; 曹迪1, 2 ; 冯建明2, 3 ; 郭绍贵2 ;宫国义2 ,伊鸿平4 ; 吴明珠4 ;张海英2*   

  1. (1 东北农业大学生命科学院, 哈尔滨150030; 2 北京市农林科学院蔬菜研究中心, 北京 100097; 3 甘肃农业大学农学院, 兰州730070; 4 新疆农业科学院哈密瓜研究中心, 乌鲁木齐830000)
  • 收稿日期:2009-04-28 修回日期:2009-10-09 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 张海英

Construction of Permanent Genetic Map and Comparative Analysis of Xinjiang Hami Melon 〔Cucumis melo L . ssp. melo convar. ameri ( Pang. ) Greb. 〕

LU Fang1, 2 ; XU Yong2 ; ZHAO Yue1 ; CAO Di1, 2 ; FENG Jian-ming2, 3 ; GUO Shao-gui2 ; GONG Guo-yi2 ; Hong-ping4 ; WU Ming-zhu4 ; ZHANG Hai-ying2*   

  1. (1L ife Science College of Northeast Agriculture University, Harbin 150030, China; 2Beijing Vegetable Research Center, Beijing Academy Agricultural and Forestry Science, Beijing 100097, China; 3Agronomy College of Gansu Agricultural University,Lanzhou 730070, China; 4Hami Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China)
  • Received:2009-04-28 Revised:2009-10-09 Online:2009-12-25 Published:2009-12-25

摘要: 新疆哈密瓜〔Cucumis melo L. ssp. melo convar. ameri ( Pang. ) Greb. 〕是我国特有的栽培甜瓜种质资源, 具有不同于网纹甜瓜〔Cucumis melo L. ssp. melo convar. cantalupa ( Pang. ) Greb. 〕的独立遗传背景。以新疆哈密瓜和网纹甜瓜为亲本构建F2 S6作图群体, 采用SSR标记技术获得同时具有哈密瓜和网纹甜瓜遗传背景的永久遗传图谱。图谱由22个连锁群组成, 包括201个SSR标记, 1个白粉病抗性基因标记, 覆盖基因组长度977.153 cM, 标记间的平均距离4.837 cM。本图谱中的17个连锁群与Fernandez- Silva
等构建的网纹甜瓜遗传背景图谱具有线性关系, 两张图谱共有46个相同SSR标记。

关键词: 甜瓜, SSR, 遗传图谱, 比较作图

Abstract: Xinjiang Hami melon, with independent and different genetic background of cantaloupe, is a unique cultivar in China. A set of populations were developed from a cross between the cantaloupe [ Cucumis melo L. ssp. melo convar. cantalupa ( Pang. ) Greb. ] and the Xinjiang Hami melon [ Cucumis melo L.
ssp. melo convar. ameri ( Pang. ) Greb. ]. Simple sequence repeat ( SSR) markers were used to construct permanent genetic map with the F2 S6 populations. The map consisted of 22 linkage groups, contained 201 SSR
markers and 1 morphological trait, it spanned a total genetic distance of 977.153 cM with an average map interval of 4.837 cM between two markers. Seventeen linkage groups in the map were collinear with cantaloupe genetic map, 46 SSR markers were common in two maps. The construction of Xinjiang Hami melon SSR map laid foundation for the continuation of the international integration of melon common genetic map and comparative study.

Key words: melon, Cucumis melo L., SSR marker, genetic map, comparative mapping

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