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园艺学报 ›› 2003, Vol. 30 ›› Issue (03): 319-321.

• 研究报告 • 上一篇    下一篇

甲基紫精滴注法快速衡量香蕉抗氧化能力的初步试验

柯德森;徐志防;孙谷畴   

  1. (中国科学院华南植物研究所,广州510650)
  • 收稿日期:2002-06-25 修回日期:2002-12-11 出版日期:2003-06-25 发布日期:2003-06-25

A Preliminary Study to Evaluate the Antioxidative Capacity of Banana by Comparing the Diameter of Methyl Viologen Speckles

Ke Desen;Xu Zhifang; Sun Guchou   

  1. (South China Institute of Botany, The Chinese Acodemy of Sciences,Guangzhou 510650,China)
  • Received:2002-06-25 Revised:2002-12-11 Online:2003-06-25 Published:2003-06-25

摘要: 滴注在香蕉(Musa paradisiaca var.sapientum)叶片表面上的甲基紫精(Methyl Viologen, 0.25 mmol·L-1 )液滴,经过50 μmol·m-2 ·s-1 光照10 h,可在滴注位点周围形成明显的褐色损伤斑点。通过测定香蕉叶片主要抗氧化酶SOD和CAT活性,发现褐斑直径与SOD和CAT活性呈明显的负相关,说明甲基紫精褐斑直径与香蕉抗氧化能力之间具有密切的关系。外源活性氧处理引起的叶片膜脂过氧化损伤与甲基紫精褐斑直径呈正相关,表明利用甲基紫精滴注法快速衡量香蕉抗氧化能力的可行性。

关键词: 香蕉, 甲基紫精, 抗氧化能力, 活性氧

Abstract: After 10 h illumination under the light of 50 μmol·m-2 ·s-1,obvious speckles could be formed in the place where the Methyl Viologen was dripped as little blobs on the surface of banana leaves. By determining the activity of two chief antioxidative enzymes,superoxide dismutase(SOD)and catalase(CAT),a significant reverse linear relationship was found between the diameter of Methyl Viologen speckle and the two enzymes activities, showing the close relationship between the speckle diam eter and the antioxidative capacity in bananas. Exogenous active oxygen species could induce the damage such as peroxidafion of membrane lipid in banana leaves , the damaging degree is closely related with the diam eter of Methyl Viologen speckles,this result indicated that the rapid method to evaluate an tioxidation capacity in bananas was feasible.

Key words: Banana, Methyl Viologen, Antioxidative capacity, Reactive oxygen species

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