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园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 612-614.

• 研究报告 • 上一篇    下一篇

梅单瓣复瓣花的相关分子标记初探

高志红1,2;韩振海1;章镇2*;张玉明2   

  1. (1中国农业大学园艺植物研究所, 北京100094 ;2 南京农业大学园艺学院, 南京210095)
  • 收稿日期:2002-11-29 修回日期:2003-02-21 出版日期:2003-10-25 发布日期:2003-10-25
  • 通讯作者: 章镇

Identification of RAPD and SCAR Markers Linked to the Single2double FlowerGene in Japanese Apricot ( Prunus mume Sieb. et Zucc. )

Gao Zhihong1,2;Han Zhenhai1;Zhang Zhen2;Zhang Yuming2   

  1. (1 Institute for Horticultural Plant,China Agricultural University,Beijing 100094 , China ; 2 College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)
  • Received:2002-11-29 Revised:2003-02-21 Online:2003-10-25 Published:2003-10-25
  • Contact: Zhang Zhen

摘要: 应用RAPD分子标记技术和SCAR 分子标记技术研究了梅单瓣花与复瓣花相关的分子标记。结
果表明从34 个随机引物中筛选到一个多态性引物OPP01 , 扩增出只有单瓣花类型具有的分子量为1899 bp的DNA 特异片段, 即OPP01-DS-1899 ; 根据该序列设计大小分别为25 bp 和24bp 的一对引物SSD-1 和SSD-2 ,将RAPD 标记转为SCAR 标记, 该标记大小为1079 bp 。

关键词: 梅, 复瓣, 单瓣, 分子标记

Abstract: Randomly amplified polymorphic DNA ( RAPD) and sequence characterized amplified region (SCAR) marker were performed to detect markers linked to the single double flower in Japanese apricot ( Prunus mume Sieb. et Zucc. ) . Primer OPP01 was identified from 34 10-mer primers examined. The fragment was cloned and sequenced as 1899 base pairs. According to the sequence , one primer pairs were designed to convert the RAPD marker to SCAR marker. The length of the amplified fragment of SCAR marker was 1079 base pairs.

Key words: Japanese apricot, Double flower, Single flower, Molecular marker

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