关键词: 樱桃, 不定根, 胚性愈伤组织, 初生体细胞胚, 不定芽, 再生

Abstract: Plant regeneration was obtained on embryogenic callus ,which derived from in vitro adventitious roots of a Chinese cherry( Prunus pseudocerasus) cultivar‘Duiyingtao’,either by adventitious bud proliferation or primary somatic embryogenesis. Embryogenic callus were induced from root segments on medium MS + NAA 1.0 mg/ L +BA 0.05 mg/ L + IBA 0.05 mg/ L ,with the frequency of 54.2 %.Adventitious bus proliferation emerged on all embryogenic callus on medium MS + NAA 1.0 mg/ L +BA 0.5 mg/ L ,the buds developed on mediumMS +BA 0.5 mg/ L + IBA 0.1 mg/ L ,and rooted on medium MS + NAA 0.02 mg/ L + IBA 0.02 mg/ L by 100 %. Primary somatic embryos and adventitious bud were induced from intact root systems simultaneously on medium MS +2 ,4-D 1-3 mg/L +BA 0.5 mg/ L , with 5 - 25 somatic embryos and 3 - 22 adventitious buds per root explant . 100 % of the somatic embryos developed into intact plantlets when transferred to medium MS +BA 0.5 mg/ L + IBA 0.1 mg/ L.

Key words: Cherry, Adventitious roots, Embryogenic callus, Primary somatic embryos, Adventitious buds, Regeneration