关键词: 月季, 小分子热激蛋白, 非生物胁迫, 抗性, 生理功能

Abstract: Rchsp1718 cloned from Chinese rose belongs to a cytosolic class I sHSP, and it is induced under different stress. In order to study the physiological function of Rchsp1718, we subcloned the ORF of Rchsp1718 recombined after 35S: p romoter into DH5α, then transformed into plant gene expression vector Agrobaterium rumefaciens, leaf disc of tobacco was infected by Agrobaterium and three transformants were attained. We assessed the phenotype, relative electrical leakage, proline content, water lose rate under high temperature, osmotic stress, and salt stress respectively, and the transcrip t levels of P5CS gene was also investigated by semi-quantitative RT2PCR. Results showed that transgenic line showed lower relative electrical leakage, more proline content, lower water lose rate, and the expressed of P5CS was elevated. Compared
with the non-transgenic lines, transformed ones showed obvious resistance to high temperature and osmotic stress. The possible mechanism to different abiotic stresswas also discussed.

Key words: Rosa chinensis, small heat shock protein, abiotic stress, resistance, physiological function