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园艺学报 ›› 2003, Vol. 30 ›› Issue (5): 530-534.

• 研究论文 • 上一篇    下一篇

低温对甘蓝逆境生理指标和蛋白质磷酸化的影响

吴能表1,2;吴峻岩1;朱利泉1;王小佳1*   

  1. (1 西南农业大学园艺系, 重庆400716; 2西南师范大学生命科学学院, 重庆400715)
  • 收稿日期:2002-12-13 修回日期:2003-02-24 出版日期:2003-10-25 发布日期:2003-10-25

Effects of Low Temperature Stress on Physiological Index and Protein Phospho-rylation in Brassica oleracea L.

Wu Nengbiao1,2;Wu Junyan1;Zhu Liquan1;Wang Xiaojia1   

  1. (1 Horticulture Department, South-West Agriculture University,Chongqing 400716,China ; 2 School of Life Science,South-West Normal University, Chongqing 400715 , China)
  • Received:2002-12-13 Revised:2003-02-24 Online:2003-10-25 Published:2003-10-25

摘要: 研究表明, 低温(5 ℃) 逆境处理0~30 min 甘蓝体内的MDA、脯氨酸、可溶性糖、ABA 含量
和PK活性等指标在较短的时间内都有明显上升, 然后下降。ABA 含量和PK活性的峰值早于其他指标, 同时低温逆境引起的PK活性对Ca2+ 的存在有明显的依赖性, Mn2+ 、Mg2+ 对其活性也有明显影响, 且这两种离子的影响机制可能不同。低温逆境信号引起22.9 kD 的蛋白质磷酸化。

关键词: 甘蓝, 温度胁迫, 蛋白激酶活性, Ca2+

Abstract: The contents of MDA , proline , soluble sugar , ABA , and the activity of PKin Brassica oleracea L. rose remarkably in a short time at 5 ℃, then went down. And the peak of ABA content and the PK activity were prior to other indexes. Low temperature , first of all , caused the activity of PKand the content of endogenesis ABA increase through some signal matter. Then , activated or prohibited some genes expressing , and at last it caused the content of other matter rising remarkably. The activity of PK had an apparently reliance on Ca2+ Mn2+ and Mg2+ had obvious effect on the PKactivity , too. The mechanismof the two ions to PK might be different . A kind of phosphoprotein (22.9 kD) caused by low temperature stress was separated

Key words: Brassica oleracea L., Temperature stress, The activity of PK, Ca2+

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