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园艺学报 ›› 2003, Vol. 30 ›› Issue (03): 272-276.

• 研究论文 • 上一篇    下一篇

龙眼品种(系)遗传多样性及亲缘关系的AFLP分析

易干军1;谭卫萍2; 霍合强1;张秋明2;李建光1;周碧容1   

  1. (1广东省农业科学院果树研究所,广州 510640; 2湖南农业大学,长沙 410128)
  • 收稿日期:2002-12-11 修回日期:2003-04-17 出版日期:2003-06-25 发布日期:2003-06-25

Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP
Analysis

Yi Ganjun1; Tan Weiping2; Huo Heqiang1; Zhang Qiuming2; Li Jianguang1; Zhou Birong1
  

  1. ( 1Institute of Fruit Tree Research,Guangdong Academy of Agricaltural Sciences, Guangzhou 510640,China; 2Hunan Agricultural University,Changsha 410128,China)
  • Received:2002-12-11 Revised:2003-04-17 Online:2003-06-25 Published:2003-06-25

摘要: 选用两对引物组合EcoRⅠ ACT+MseⅠ CTT和 EcoRⅠ ACT+MseⅠ CTC对46份龙眼材料进行AFLP分析,共得到扩增位点111个,其中多态性位点103个,多态性比例达92.69%,区分率达100%。对AFLP扩增结果进行聚类分析,根据相似系数0.76的水平可将46份龙眼品种(系)分为11个品种群。供试各品种多态性比例介于0.2703和0.4955间。福建东壁与广东东壁应为不同品系;早熟一号为一个新的品系。

关键词: 龙眼, AFLP, 遗传多样性, 亲缘关系

Abstract:

The study is mainly on the genetic diversity and relationship of Longan (Dimocarpus longan Lour.) cultivars by applying AFLP (Amplified Fragment Length Polymorphism). 103 polymorphic distinct bands were obtained with two pairs of primers(EcoRⅠACT+Mse ⅠCTT and EcoR ⅠACT+Mse Ⅰ CTC),which were enough to distinguish the 46 cultivars from each other. UPGMA analysis showed that 46 cultivars were divided into 11 groups.The ratio of polymorphic rate varied from 0.2703 to 0.4955.The result showed that the Fujian Dongbi
was diferent from Guangdong Dongbi and they were different types from Kaiyuansi Dongbi.Zaoshu 1 was supposed to be a new type.The results supplied theoretical basis for cultivar classification.

Key words: Longan, AFLP, Genetic diversity, Relationship

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