关键词: 苹果, 柱型苹果, 柱型基因, 遗传分析, ISSR 标记

Abstract: In the present paper, cultivated apple ‘Fuji’(standard growth type),‘Waltz’(or‘Telemon’,columnar growth type) and seedlings in their progeny with standard and columnar types were used to establish the ISSR inter-simple sequence repeat) analyses in apple. ISSR markers were also used for genetic analysis of the columnar gene. The results showed that 80 %primers produced good amplification in 20μL reaction volume include 1U Taq DNA polymerase , 2.5 mmol ·L^-1 Mg²⁺ , 20 ng DNA and 0.2 μmol ·L ^-1 primer with the annealing
temperature 52 ℃. Adjusting the concentration of the template DNA , primers and the annealing temperature can optimize the ISSR - PCR amplification. 35 ISSR markers were obtained from the screened 65 primers. Among them 33 ISSR markers , which displayed 1∶1 segregant ratio in correspondence with the genetic segregation of Co gene, could be used for genetic analysis of the Co gene in apple.

Key words: Apple ( Malus ×domestica), Columnar apple, Co gene, ISSR marker, Genetic analysis