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园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 321-323.

• 研究报告 • 上一篇    下一篇

茄子抗青枯病基因的RAPD标记研究

朱华武1;姚元干2;刘志敏1;杨建国2;陈惠明2;邹学校2   

  1. (1 湖南农业大学园艺园林学院, 长沙410128; 2 湖南省蔬菜研究所, 长沙410125)
  • 收稿日期:2004-05-20 修回日期:2004-10-10 出版日期:2005-04-25 发布日期:2005-04-25

Studies on RAPD Marker of Bacterial Wilt Resistance Gene in Eggplant( Solanum melongena )

Zhu Huawu1;Yao Yuangan2;Liu Zhimin1;Yang Jianguo2;Chen Huiming2;Zou Xuexiao2   

  1. ( 1College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China; 2Hunan Vegetable Research Institute, Changsha 410125, China)
  • Received:2004-05-20 Revised:2004-10-10 Online:2005-04-25 Published:2005-04-25

摘要: 为探明茄子抗青枯病的遗传机制, 以高感青枯病品种北京六叶茄064, 高抗青枯病半栽培种马来西亚S3及其F2 代为材料, 用RAPD技术对供试材料的抗青枯病基因进行了研究。结果表明: 通过300个随机引物的PCR扩增分析, 发现15.6%的引物在双亲间表现出多态性, 找到了一个与茄子抗青枯病亲本S3中的抗病基因紧密连锁的分子标记S264780 (该引物的碱基序列为CAGAGCGGA) , 该标记与S3的抗病基因的交换值为4.32% , 遗传距离为4.33 cM。

关键词: 茄子, 抗青枯病基因, 分子标记

Abstract: To p robe into the genetic resistant law to bacterialwilt (BW) in eggp lant, the genetic studies to BW of 064 (Beijing six-leaf eggp lant) , S3 ( highly resistant half-cultivated species of Malasia) and 064 ×S3 F2 populations is carried out on RAPD marker. 300 decamer p rimerswith arbitrary sequences are chosen for
polymerase chain reaction amplication. It is found that the polymorphism between the parents could be detected by 15.6% of the primer; one RAPD marker S264780 ( the sequence of the primer: CAGAAGCGGA) tightly links to the resistance gene of S3 with the overcross value of 4.32% and genetic distance of 4.33 cM.

Key words: Solanum melongena, Bacterial wilt resistance gene, RAPD

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