大葱成熟胚离体再生过程中内源激素的变化

园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 318-320.

大葱成熟胚离体再生过程中内源激素的变化

盛艳萍¹;杨建平¹;张松¹;齐宪磊²;李新成³

(¹山东农业大学园艺学院, 泰安271018; ² 山东农业大学植物保护学院, 泰安271018; ³ 泰安市菜篮子科技园, 泰安271018)

收稿日期:2004-04-01 修回日期:2004-06-21 出版日期:2005-04-25 发布日期:2005-04-25

Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture

Sheng Yanping¹;Yang Jianping¹;Zhang Song¹;Qi Xianlei²;Li Xincheng³

(¹College of Horticulture, Shandong Agricultural University, Tai'an 271018, China; ²College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China; ³ Tai'an Institute of Vegetable Basket, Tai'an 271018, China)

Received:2004-04-01 Revised:2004-06-21 Online:2005-04-25 Published:2005-04-25

摘要/Abstract

摘要： 对大葱成熟胚的愈伤组织诱导及分化过程进行了观察, 并对再生过程中内源激素水平进行了测定。研究结果表明: 大葱愈伤组织诱导过程中, ZR在3 d时含量达峰值, 可能是导致6 d时增长率最高的主要原因, ABA在此过程中可能为负调控因子。GA₃ 在愈伤组织分化过程中的含量明显高于继代过程,说明其对大葱愈伤组织的分化有重要作用。适当高的6-BA与NAA比值有利于芽的分化, 但是细胞分裂素类浓度过高时则会对不定芽造成伤害。

关键词: 大葱, 成熟胚, 愈伤组织诱导, 分化, 内源激素

Abstract: The callus induction and p lant regeneration from Allium fistulosum L. mature embryos culture were studied in A. f istulosum L. and the endogenous hormone changes in this course were analyzed. The callus growth rate reached the highest point at the 6 th day after culturing, which might be caused by the maximum concentration of ZR at the 3 rd day. ABA was a negative regulation factor in the callus induction. The concentration of GA₃ during callus induction wasmuch higher than that in the multip lication course, which indicated GA₃ was very important in the differentiation period. The appropriate proportion between 6-BA and NAA was beneficial to the shoot differentiation of A. fistulosum L. , but there was side effect to shoot regeneration if CTK concentration was too high.

Key words: Allium fistulosum L., Mature embryos, Callus induction, Differentiation, Endogenous hormones

中图分类号:

S633.1

盛艳萍;杨建平;张松;齐宪磊;李新成. 大葱成熟胚离体再生过程中内源激素的变化[J]. 园艺学报, 2005, 32(02): 318-320.

Sheng Yanping;Yang Jianping;Zhang Song;Qi Xianlei;Li Xincheng. Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture[J]. ACTA HORTICULTURAE SINICA, 2005, 32(02): 318-320.

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