'寒富'苹果自花结实性分析及S基因型鉴定

园艺学报 ›› 2008, Vol. 35 ›› Issue (4): 475-480.

'寒富'苹果自花结实性分析及S基因型鉴定

白松龄¹;伊凯²;刘国成³;韩振海¹;许雪峰¹;王冬梅²;李天忠^1*

（¹中国农业大学园艺植物研究所，北京 100094；²辽宁省果树研究所，辽宁熊岳 115009；3沈阳农业大学园艺学院，辽宁沈阳 110161）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 李天忠

Analysis of the Self-fruitfulness of 'Hanfu' Apple and Identification of the S-Genotypes

BAI Song-ling¹, YI Kai², LIU Guo-cheng³, HAN Zhen-hai¹, XU Xue-feng¹, Wang Dong-mei², and LI Tian-zhong^1*

（¹Institute of Horticultural Plants，China Agricultural University, Beijing 100094, China；²Liaoning Research Institute of Pomology, Xiongyue, Liaoning 115009, China；³College of Horticulture，Shenyang Agricultural University, Shenyang, Liaoning 110161, China）

Received:1900-01-01 Revised:1900-01-01 Online:2008-04-25 Published:2008-04-25
Contact: LI Tian-zhong

摘要/Abstract

摘要：

本研究以苹果‘寒富’品种及其亲本为试材，研究其自交亲和性并鉴定其S基因型。田间授粉试验结果显示，‘寒富’品种表现为自花结实，其母本‘东光’品种亦自花结实；而父本‘富士’品种自花授粉结实率极低，初步推断‘寒富’品种的自花结实性可能遗传自其母本‘东光’。利用PCR技术鉴定'寒富'品种S基因型为S1S9。由于‘东光’品种存在同名异物问题，本研究除日本‘东光’品种外，对辽宁省果树研究所、吉林省果树研究所、沈阳农业大学等单位保存的中国'东光'品种的S基因型进行了鉴定，发现除辽宁省果树研究所保存的'东光'品种S基因型为S2S19，与‘寒富’品种无亲缘关系外，其它两个单位保存的'东光'品种中均存在S9基因型，可能为'寒富'杂交育种时的原始母本。

关键词: 苹果, ‘寒富’, 自花结实性, S基因型, 鉴定

Abstract:

'Hanfu' Apple cultivar(Malus×domestica) and its parents were used in this research to study its self-compatibility and identify their S-genotypes. The pollination test in the field showed that the 'Hanfu' is self-compatible, and its female parent 'Dongguang' is also self-compatible, but its male parent 'Fuji' is self-sterile as known. The S-genotype of 'Hanfu' was identified as S1S9 by the PCR-based method. Also, the S-genotypes of all 'Dongguang' cultivars which are homonym were identified except the 'Toko' which came from Japanese and was named as 'Dongguang' in Chinese. The results showed the S-genotype of 'Dongguang' from Liaoning Research Institute of Pomology is S2S19, showing no relationship with 'Hanfu', and the S-Genotypes of 'Dongguang' cultivars from Shenyang Agricultural University, and Jilin Research Institute of Pomology contain S9 allele, suggesting they are probably the female parent of 'Hanfu'.

Key words: Apple, 'Hanfu', Self-fruitfulness, S-genotype, Identification

中图分类号:

S 661

白松龄;伊凯;刘国成;韩振海;许雪峰;王冬梅;李天忠 . '寒富'苹果自花结实性分析及S基因型鉴定[J]. 园艺学报, 2008, 35(4): 475-480.

BAI Song-ling;YI Kai;LIU Guo-cheng;HAN Zhen-hai;XU Xue-feng;Wang Dong-mei;and LI Tian-zhong. Analysis of the Self-fruitfulness of 'Hanfu' Apple and Identification of the S-Genotypes[J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 475-480.

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