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园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1607-1612.

• 蔬菜 • 上一篇    下一篇

番茄CONSTANS类似基因的克隆与表达分析

叶雪凌1,刘志勇2,王孝宣2,杜永臣2*,国艳梅2

  

  1. (1首都师范大学生命科学学院,北京100037;2中国农业科学院蔬菜花卉研究所,北京100081)
  • 收稿日期:2008-06-02 修回日期:2008-09-11 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 杜永臣

Molecular cloning and Expression Analysis of a CONSTANS-Like Gene, SlCO1, from Solanum lycopersicum

Ye Xue-ling1;Liu Zhi-yong2;Wang Xiao-xuan2;Du Yong-chen2*;and Guo Yan-mei2   

  1. (1College of Life Science, Capital Normal University, Beijing 100037, China;2Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2008-06-02 Revised:2008-09-11 Online:2008-11-25 Published:2008-11-25
  • Contact: Du Yong-chen

摘要:

以一个受生物钟调节的番茄转录衍生片段(transcription-drived fragments,TDF)为信息探针,通过在NCBI EST数据库中进行电子延伸和RT- PCR验证,获得了番茄CO类似基因的cDNA全长序列,命名为SlCO1(GenBank登录号,EU636698)。SlCO1蛋白含有386个氨基酸,氨基端存在两个B-box型锌指结构,羧基端含有一个TTC结构域。SlCO1蛋白与CO类蛋白的氨基酸一致性为34 %~46 %,但在B-box锌指和CCT结构域处高度保守。基因组序列包含1个535 bp的内含子,位于B-box锌指和CCT结构域之间。表达谱分析表明,SlCO1在叶片中优势表达,在根和茎中表达较弱。SlCO1的克隆为了解CO类似基因在番茄开花中的作用奠定了基础。

关键词: 番茄, 开花时间, CONSTANS类似基因

Abstract: Derived from tomato and influenced by circadian clock, a TDF(transcription-drived fragments, TDF)was used as a seed sequence for searching homologous ESTs through electronical elongation in NCBI database. A full-length CONSTANS-Like gene designed as SlCO1(GenBank accession number, EU636698), is cloned using RT-PCR. The deduced SlCO1 protein consisting of 386 amino acid residues contains two B-box zinc finger motifs near the amino terminus and a conserved CCT domain near the carboxy terminus. Moreover, a 535 bp intron is located between the B-box and CCT domain. Although SlCO1 proteins shares 34 %~46 % identity with other CO proteins, the deduced amino acid sequence is highly conserved in the B-box and CCT domain. SlCO1 expresses specifically and strongly in leaf, but weakly in roots and stems. Cloning of SlCO1 will provide a scientific basis for understanding the role of CONSTANS-like genes in tomato flowering process.

Key words: Tomato, Solanum lycopersicum, flowering time, CONSTANS-like gene

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