关键词: 转基因, 枳橙, rol基因, RT-PCR, 形态特征, Pn, 内源激素

Abstract: Molecular identification, physiology evaluation and morphological observation of the rolA, rolB and rolC transgenic citrange clones (B, D, E) were conducted. PCR analysis indicated that the rolA, rolB, rolC geneswere integrated into the citrange genome. The expression of the transgenes nptⅡ and rolC was detected by using RT-PCR; while the transgenes rolA and rolB did not show any RT-PCR product, and it was not clear for the factors related to their expression. In comparison with the control plants, the rol transgenic plant showed evident dwarfing characteristics, such as only half of the control in plant height, shorter internodal length, weaker apical dominance with more lateral branches, smaller leaf area, and higher net photosynthesis rate. Higher contents of the endogenous GA3 , IAA and zeatin were found in the middle and basal parts of the transgenic shoots than the control, this could be one of the factors responsible for the above described morphological modifications.

Key words: Transgenic, Citrange, rol gene, RT-PCR, Morphology, Pn, Endogenous hormone