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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 571-576.

• 研究论文 • 上一篇    下一篇

东方百合鳞茎冷藏解除休眠的养分代谢和酶活性变化

夏宜平1;黄春辉1;何桂芳1, 2;郑慧俊1, 3

  

  1. (1 浙江大学农业与生物技术学院园艺系, 浙江杭州310029; 2 青海大学农牧学院农学系, 青海西宁810016; 3 杭州职业技术学院艺术系, 浙江杭州310018)
  • 收稿日期:2005-09-19 修回日期:2006-01-05 出版日期:2006-06-25 发布日期:2006-06-25

Changes of Carbohydrates Metabolism and Enzymes Activities in Low Temperature Storage for Bulbs of Lilium Oriental Hybrids

Xia Yiping1;Huang Chunhui1;He Guifang1, 2;Zheng Huijun1, 3   

  1. ( 1Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029,China; 2Husbandry and Agronomy College of Qinghai University, Xining, Qinghai 810016, China; 3Arts Department, Hangzhou College of Vocation Technology, Hangzhou, Zhejiang 310018, China)
  • Received:2005-09-19 Revised:2006-01-05 Online:2006-06-25 Published:2006-06-25

摘要: 以自繁的东方百合鳞茎为材料, 研究在2℃、5℃和8℃条件下, 鳞片中淀粉、可溶性总糖、还原糖含量和几种酶活性的变化。在8周的冷藏期内, 淀粉含量持续明显下降, 可溶性糖和还原糖含量增加, CAT、POD、SOD酶的活性在冷藏第1周下降极显著, 而α - 淀粉酶活性在第3周出现峰值, 自冷藏第4周后4种代谢酶活性均处于低水平。本试验中, 不同低温处理间的差异不大, 冷藏第7周后可解除休眠。

关键词: 东方百合, 鳞茎, 冷藏, 解除休眠, 养分代谢, 酶活性

Abstract: Carbohydrate contents and related enzymes activities were performed to investigate the effect of low temperature storage on the bulbspdormancy of Oriental Lily‘Sorbonne’. Bulbs were stored in moist peat moss for 8 weeks at 2℃, 5℃ and 8℃, respectively. The starch contents decreased continuously in interior and exterior scales, while the contents of total soluble carbohydrates and reducing sugar were increased. Activities of CAT, POD and SOD declined significantly, especially in the firstweek of cold storage. A peak value in α-amylase activity appeared in the 3rd week. Low activity levels of all enzymes presented after the 4th week. The results indicated that no obvious difference between the three low temperatures, and overcoming dormancyin bulbs of oriental lily‘Sorbonne’started in the 7th week.

Key words: Oriental lily, Bulb, Cold storage, Overcoming dormancy, Carbohydrate metabolism, Enzyme activity