利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达

园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 544-548.

利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达

康俊根^{1, 2};王晓武^1*;张国裕¹;张延国¹;娄　平¹;方智远¹

(¹中国农业科学院蔬菜花卉研究所, 北京100081; ²山西省农业科学院蔬菜研究所, 山西太原030031)

收稿日期:2005-07-07 修回日期:2006-01-17 出版日期:2006-06-25 发布日期:2006-06-25

Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines

Kang Jungen^{1, 2};Wang Xiaowu^1*;Zhang Guoyu¹;Zhang Yanguo¹;Lou Ping¹;Fang Zhiyuan¹

(¹ Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Institute of Vegetable, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China)

Received:2005-07-07 Revised:2006-01-17 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 通过cDNA-AFLP技术分别对4种具有不同败育时期特征的甘蓝雄性不育材料和遗传背景一致的可育材料进行分析, 揭示了4种不育类型育性相关基因的时序性表达特征, 并对4种育性相关基因分别选取1个转录表达片段(TDFs) 克隆测序。结果表明, 获得的113条不同表达方式TDFs可分为12种模式: 其中按照不同雄性不育败育顺序表达中断的有A、B、C、D 4种模式, 分别代表造孢细胞期、花粉母细胞期、四分体前期和四分体后期发育中断的育性相关基因。这4种表达模式包括76条TDFs, 占总TDFs的67.25%。结合发育中断模型和序列分析结果, 推测糖基水解酶家族基因、具有VQ结构域的基因、富含甘氨酸蛋白和促进游离小孢子解离的果胶裂解酶基因可能分别参与上述花药发育4个时期雄性育性的建成。

关键词: 甘蓝, 花药发育, cDNA-AFLP, 基因表达

Abstract: Elucidating the gene expression in anther development is one of the key steps to develop new types of male sterility. Four types of male sterile cabbage lines and their corresponding fertile lines were used to analyze the gene expression by cDNA-AFLP. In total, we got 113 differentially exp ressed TDFs, which were
classified into 12 group s. Among them, 76 TDFs (67.25% of the total) were classified into group A, B, C and D, which were sequentially interrupted according to the different sterile stages in four male sterile lines. Sequence analysis of representative TDFs from each group showed that glycosyl hydrolase family 1 protein, VQ motif-containing protein, glycine-rich protein and pectatelyase family protein possibly involved in sporogenesis cell, pollen mother cell, pretetrad and post-tetrad stage male development respectively.

Key words: Cabbage, Anther development, cDNA-AFLP, Gene expression

康俊根;;王晓武;张国裕;张延国;娄　平;方智远. 利用cDNA-AFLP检测甘蓝雄性不育相关基因的时序性表达[J]. 园艺学报, 2006, 33(3): 544-548.

Kang Jungen;;Wang Xiaowu;Zhang Guoyu;Zhang Yanguo;Lou Ping;Fang Zhiyuan. Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 544-548.

[1]	王晓晨, 聂子页, 刘先菊, 段伟, 范培格, 梁振昌, . 脱落酸对‘京香玉’葡萄果实单萜物质合成的影响[J]. 园艺学报, 2023, 50(2): 237-249.
[2]	韩睿, 钟雄辉, 陈登辉, 崔建, 乐祥庆, 颉建明, 康俊根, . 黑腐病菌效应因子XopR的甘蓝靶标基因BobHLH34的克隆及功能分析[J]. 园艺学报, 2023, 50(2): 319-330.
[3]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
[4]	崔建, 钟雄辉, 刘泽慈, 陈登辉, 李海龙, 韩睿, 乐祥庆, 康俊根, 王超. 结球甘蓝染色体片段替换系构建[J]. 园艺学报, 2023, 50(1): 65-78.
[5]	吕红豪, 杨丽梅, 方智远, 张扬勇, 庄木, 刘玉梅, 王勇, 季家磊, 李占省, 韩风庆. 春甘蓝新品种‘中甘27’和‘中甘28’[J]. 园艺学报, 2022, 49(S1): 63-64.
[6]	周娜, 陶伟林, 陆景伟, 郑阳, 胡燕, 潘晓雪. 甘蓝新品种‘渝园春印’[J]. 园艺学报, 2022, 49(S1): 65-66.
[7]	李强, 蔡玉梅, 苏彦宾, 王英, 顾丽嫱, 赵玉倩. 中早熟甘蓝新品种‘劲绿60’[J]. 园艺学报, 2022, 49(8): 1835-1836.
[8]	张秋悦, 刘昌来, 于晓晶, 杨甲定, 封超年. 盐胁迫条件下杜梨叶片差异表达基因qRT-PCR内参基因筛选[J]. 园艺学报, 2022, 49(7): 1557-1570.
[9]	陈道宗, 刘镒, 沈文杰, 朱博, 谭晨. 白菜、甘蓝和甘蓝型油菜PAP1/2同源基因的鉴定及分析[J]. 园艺学报, 2022, 49(6): 1301-1312.
[10]	李亚梅, 马福利, 张山奇, 黄锦秋, 陈梦婷, 周军永, 孙其宝, 孙俊. 酸枣愈伤组织转化体系构建及在ZjBRC1调控ZjYUCCA表达中的应用[J]. 园艺学报, 2022, 49(4): 749-757.
[11]	张瑞, 张夏燚, 赵婷, 王双成, 张仲兴, 刘博, 张德, 王延秀. 基于转录组分析垂丝海棠响应盐碱胁迫的分子机制[J]. 园艺学报, 2022, 49(2): 237-251.
[12]	周至铭, 杨佳宝, 张程, 曾令露, 孟晚秋, 孙黎. 向日葵LACS家族鉴定及响应非生物胁迫表达分析[J]. 园艺学报, 2022, 49(2): 352-364.
[13]	乔军, 王利英, 刘婧, 李素文. 基于转录组测序的茄子萼下果色光敏相关基因表达分析[J]. 园艺学报, 2022, 49(11): 2347-2356.
[14]	叶广继, 郑贞贞, 纳添仓, 王舰. 马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析[J]. 园艺学报, 2022, 49(11): 2357-2366.
[15]	侯天泽, 易双双, 张志群, 王健, 李崇晖. 秋石斛RT-qPCR内参基因的筛选与验证[J]. 园艺学报, 2022, 49(11): 2489-2501.