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园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1427-1442.

• 果树 • 上一篇    下一篇

番木瓜eIF(iso)4E基因克隆及其结构和表达分析

言普;沈文涛; 高新征;李亚丽;周鹏*   

  1. (中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点实验室, 海口571101)
  • 收稿日期:2009-05-25 修回日期:2009-08-24 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 周鹏

e eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzlingin Papaya

YAN Pu; SHEN Wen-tao.GAO Xin-zheng;LI Ya-li;ZHOU Peng*   

  1. (Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture,institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou571101, China)
  • Received:2009-05-25 Revised:2009-08-24 Online:2009-10-25 Published:2009-10-25
  • Contact: ZHOU Peng

摘要: eIF4E ( eukaryotic translation initiation factor 4E) 家族基因在多种病毒侵染植物过程中起重要作用。应用RT-PCR和RACE方法在番木瓜中克隆了cDNA全长为995 bp的eIF4E异构体基因, 命名为CP-eIF(iso)4E(GenBank登录号为FJ595992) 。其编码的蛋白含有206个氨基酸, 蛋白一级结构中的活性部位位点高度保守, 三维结构中可能的抗性位点位于帽结合区域( cap-binding pocket) 表面。半定量RT-PCR结果表明, CP-eIF ( iso) 4E在番木瓜叶片中表达较弱, 在花瓣中表达最强。

关键词: 番木瓜, eIF ( iso) 4E, 结构分析, 基因表达

Abstract: eIF4E ( eukaryotic translation initiation factor 4E) family genes play important roles against virus from infecting plants. A complete cDNA of 995 bp from papaya was cloned using the method of RT-PCR and RACE. It was a isoform eIF4E, so itwas named CP-eIF ( iso) 4E (GenBank accession No. FJ595992).
The predicted CP-eIF ( iso) 4E protein had 206 amino acids, in which the active site was high conservative in primary structure, and the predicted virus-resistance site was located on the surface of cap-binding pocket in3-D structure. Semi-quantitative RT-PCR analysis indicated that CP-eIF ( iso) 4E expression was lower in leaf and higher in petal of papaya.

Key words: papaya, eIF ( iso) 4E, structure analysis, gene expression

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