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园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1093-1095.

• 研究报告 • 上一篇    下一篇

TuMV-Nib反义基因对大白菜的遗传转化研究

于占东1, 2;赵双宜3;何启伟1*;王翠花1;牟晋华1;刘春香1;康静2   

  1. (1 山东省农业科学院蔬菜研究所, 山东济南250100; 2 吉林农业大学园艺学院, 吉林长春130118; 3 山东大学生命科学院, 山东济南250100)
  • 收稿日期:2005-10-19 修回日期:2006-01-23 出版日期:2006-10-25 发布日期:2006-10-25

Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages

Yu Zhandong1, 2;Zhao Shuangyi3;He Qiwei1*;Wang Cuihua1;Mou Jinhua1; Liu Chunxiang1;Kang Jing2   

  1. (1 Institute of Vegetable Crops, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong 250100, China; 2College of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, China; 3College of Life Sciences, Shandong University, Ji'nan,Shandong 250100, China)
  • Received:2005-10-19 Revised:2006-01-23 Online:2006-10-25 Published:2006-10-25

摘要:

以‘福山包头’大白菜子叶为试材, 以Anti2TuMV2Nib为目的基因, 利用根癌农杆菌介导法,建立了大白菜再生转化体系, 获得转化植株。经PCR-Southern杂交、RT-PCR 及ELISA 检测, 证明外源TuMV-Nib基因已整合到大白菜核基因组中并获得了表达。

关键词: 大白菜, 芜菁花叶病毒, 复制酶基因, 遗传转化

Abstract: Agrobacterium tumiefaciens-mediated regeneration system was established using Anti-TuMV-Nib gene. Cotyledons with petiole from aseptic seedlings of Chinese cabbage (Brassica campestris ssp. pekinensis‘Fushanbaotou’) were used as explants and the transgenic plants were obtained. The analysis of PCR-Southern blotting, RT-PCR and ELISA detection showed that the target gene was integrated into the Chinese cabbage genome and expressed.

Key words: Chinese cabbage, TuMV, Nib gene, Genetic transformation