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园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 47-58.

• 研究论文 • 上一篇    下一篇

西瓜重组自交系群体的AFLP 分子图谱构建

易克1;许勇2*;卢向阳1;肖浪涛1;徐向利1;宫国义2;张海英2   

  1. (1 湖南农业大学生化与发酵工程实验室, 长沙410128 ; 2 国家蔬菜工程技术研究中心, 北京100089)
  • 收稿日期:2003-04-24 修回日期:2003-09-26 出版日期:2004-02-25 发布日期:2004-02-25
  • 通讯作者: 许勇

Construction of AFLP Molecular Genetic Map for RIL Population of Watermelon

Yi Ke1;Xu Yong2*;Lu Xiang-yang1;Xiao Lang-tao1;Xu Xiang-li1;Gong Guoyi2; Zhang Hai-ying2   

  1. (1 Department of Biotechnology of Hunan Agriculture University , Changsha 410128 , China ; 2 National Engineering Research Center for Vegetables , Beijing 100089 , China)
  • Received:2003-04-24 Revised:2003-09-26 Online:2004-02-25 Published:2004-02-25
  • Contact: Xu Yong

摘要: 以可溶性固形物含量高、皮薄、感枯萎病的栽培西瓜自交系( Citrullus lanatus var. lanatus)97103 和可溶性固形物含量低、皮厚、抗病的野生西瓜种质( Citrullus lanatus var. citroides) PI296341 为亲本构建获得F2S7 的重组自交系群体, 通过AFLP 技术对该群体进行扩增, 建立了一个包括150 个标记组成的分子图谱。该图谱包括17 个连锁群, 覆盖基因组范围1240.2 cM, 两个标记间的平均图距为8.3 cM。图谱的建立对于西瓜高密度遗传图谱的构建、重要农艺性状的QTL 定位以及重要基因的图谱克隆均具有重要的参考价值。

关键词: 西瓜, AFLP 标记, RIL 群体, 连锁图谱

Abstract: A recombinant inbred lines of 117 F2S7 progenies derived from the cross 97103 (which is a cultivarwith higher total soluble solids concentration , thin rind , susceptible to Fusarium wilt disease ) ×PI296341 (which is an African wild germplasm with lower total soluble solids concentration , thick rind , resistant to Fusarium wilt disease) was used to construct a map containing 150 AFLP markers. This map consists of 17 linkage groups , covering 1240.2 cMwith an average distance of 8.3 cM between locus. It will contribute to construction of a high-density map of watermelon , QTL analysis of important agronomic traits and map-based gene clone.

Key words: Watermelon, AFLP marker, Recombinant inbred lines, Linkage map

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