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园艺学报 ›› 2004, Vol. 31 ›› Issue (3): 397-399.

• 研究报告 • 上一篇    下一篇

丛生福禄考的离体繁殖

代红艳;张志宏;高秀岩;王凤兰   

  1. (沈阳农业大学园艺学院,沈阳110161)
  • 收稿日期:2003-08-12 修回日期:2003-09-27 出版日期:2004-06-25 发布日期:2004-06-25
  • 通讯作者: 代红艳

In Vitro Propagation of Phlox subulata L.

Dai Hongyan;Zhang Zhihong;Gao Xiuyan;Wang Fenglan   

  1. (College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China)
  • Received:2003-08-12 Revised:2003-09-27 Online:2004-06-25 Published:2004-06-25

摘要: 建立了丛生福禄考离体繁殖体系。用0.1% HgCl2溶液对丛生福禄考的新生嫩茎进行消毒,
适宜的消毒时间为10 min。1 mm茎尖的成苗率达到83.3%。去顶芽的试管苗新梢比具顶芽的试管苗新梢的增殖能力高约50%。采用腋芽萌发途径,品种丹尼尔垫和多摩流的增殖系数分别达18和8。在附加IBA 0. mg/L的1/2 MS培养基上新梢的生根率达到80%。试管苗大规模移栽的成活率达90%以上。基因型显著地影响丛生福禄考离体增殖的效率。

关键词: 丛生福禄考, 离体繁殖, 茎尖, 生根

Abstract: An eficiency system of propagation jn vitro of Phlox subulata L.was developed. The optimum sterilization of young shoots was 10 minutes with 0.1% HgCl2. The rate of shoots forming from 1 mm shoot tips reached 83.3%. The multiplication cogmcient of in vitro decapitated shoot was 50% higher than
the multiplication coefficient of whole shoot.For cultivars ‘Daniel cushion’and ‘Tamaryuu’.the multiplication coeficients reached 18 and 8 respectively. On the half strength MS medium supplemented with IBA 0.2 mg/L.the rate of rooting of in vitro shoots of P.subulata L. reached 80%.The survival rate of
plants transplanted on a large scale was more than 90% .The eficiency of multiplication of Phlox subulata L.was afected significantly by genotypes.

Key words: Phlox subulata L., Propagation in vitro, Shoot tip, Rooting

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