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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 242-244.

• 研究简报 • 上一篇    下一篇

四川主栽茶品种亲缘关系的RAPD分析

王雪萍1;马炳田2*
; 齐桂年1**
;田 鸿1 ;方倡友3 ; 张泽岑1 ; 尹旭敏1
  

  1. (1 四川农业大学林学园艺学院, 四川雅安625014; 2 四川农业大学水稻研究所, 成都611130; 3 四川省雅安市雨城区农业局, 四川雅安625000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

RAPD Analysis on the Genetic Relationsh ips of Tea Cultivars Grown inSichuan

WANG Xue-ping1 ; MA Bing-tian2*
; QI Gui-nian1**; TIAN Hong1 ; FANG Chang-you3 ;ZHANG Ze-cen1 ; YIN Xu-min1
  

  1. (1 College of Forestry and Horticultural, Sichuan Agricultural University, Ya'an, Sichuan 625014, China; 2 R ice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 3 District of Yucheng Agricultural Adm inistration, Ya'an, Sichuan625000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 利用RAPD标记对四川主要产茶区具代表性的36份茶树栽培品种的亲缘关系进行分析。从
50条引物中筛选出扩增效果良好的16条, 共扩增出167条谱带, 其中, 多态性带为158条(占94.61% ) ,
遗传距离的变异范围在0.149~0.679之间。UPGMA聚类结果将36份茶树栽培品种分为3个复合组和2个
独立组, 聚类结果与茶树品种的来源有关。

关键词: 茶树, RAPD, 亲缘关系, UPGMA

Abstract: The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.
Sixteen primers selected from 50 random p rimerswere used to amp lify 36 tea samples, and a total of 167 DNA
bandswere amplified, among which 158 (94.61% ) were polymorphic. Genetic distances between the culti
vars varied from 0.149 to 0.679. UPGMA cluster analysis of 167 bands amplified by 16 p rimers was made.
The result showed that 36 tea cultivars tested could be classified into 3 comp lex group s and 2 simp le group s. It
was found that there was a certain correlation between the results and the derivation of tea cultivars.

Key words: Tea plant, Camellia sinensis, RAPD, Genetic relationship, UPGMA