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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 227-230.

• 研究简报 • 上一篇    下一篇

光和糖对非洲菊花色素苷积累及CHS、DFR 基因表达的影响

孟祥春1,2; 彭建宗1; 王小菁1*   

  1. (1 华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631; 2 广东省农业科学院果树研究所, 广州510640)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Anthocyan in Accumulation and CHS, DFR Gene Expression Regula ted byLight and Sugar in Gerbera hybrida Ray Floret

MENG Xiang-chun1,2; PENG Jian-zong1; WANG Xiao-jing1*   

  1. (1 College of Life Sciences, South China Normal University, Guangdong Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China; 2 Research Institute of Pomology, Guangdong Academ y of Agricultural Sciences, Guangzhou 510640,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 探讨了光和糖对非洲菊(Gerbera hybrida) 花序最外轮舌状花( ray floret) 花瓣着色的影响。
P2期花序和舌状花先在含有3%蔗糖和无蔗糖的固体培养基(8 g·L-1琼脂) 上进行3 d暗培养, 光照处
理不同时间后, 继续暗培养, 第9 天取样, 采用分光光度计法测定舌状花花瓣花色素苷积累; 照光后立即
取样, 以Northern-blot检测CHS、DFR基因表达的变化。研究结果表明, 短暂光照提高花色素苷积累; 光
照明显诱导CHS、DFR基因表达, 光照时间越长, 基因表达水平也越高。暗培养7 d和9 d, 光诱导基因表
达效应明显减弱。培养基中含有蔗糖时, 光强烈诱导花色素苷积累和CHS、DFR基因表达。

关键词: 非洲菊, 花色素苷, 光, 糖, 基因表达

Abstract: Effects of light and sugar on Gerbera hybrida ray floret ( rf) p igmentation were investigated.
Inflorescences or rfs at stage P2 were detached and incubated on the medium (8 g·L-1 agarwith 3% sucrose
or free of sucrose) under 14 h light /10 h dark cycles at 23 - 25℃. Cultured inflorescences and rfswere first
incubated in darkness for 3 d, then exposed to transient light for a period of time followed by an incubation in
the dark until harvesting for anthocyanin determination by spectrophotometer at 9 th d or harvested immediately
for gene exp ression detected byNorthern-blot. The results showed that transient light exposure induced antho
cyanin accumulation and the gene exp ression of CHS and DFR in rf petal after 3 d p reculture in darkness. The
longer time of light irradiation strongly enhanced the gene exp ression of CHS and DFR . Petals precultured in
darkness for 7 d and 9 d had weak response to light in gene expression. When sucrose was present in the me
dium, both anthocyanin accumulation and gene exp ression were strongly increased under light condition.

Key words: Gerbera, Anthocyanin, Light, Sugar, Gene expression