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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 101-103.

• 研究报告 • 上一篇    下一篇

诱导黄瓜直接器官发生主要影响因素的研究

侯爱菊;朱延明*;杨爱馥;张彬彬   

  1. (东北农业大学生命科学学院植物生物工程研究室, 哈尔滨150030)
  • 收稿日期:2002-02-07 修回日期:2002-06-17 出版日期:2003-02-25 发布日期:2003-02-25

Main Factors Influencing the Frequency of Direct Organogenesis of Cucumberin Vitro

Hou Aiju;Zhu Yanming;Yang Aifu;Zhang Binbin   

  1. ( Plant Bioengineering Laboratory , Life Science Institute, North East Agriculture University , Harbin 150030 , China)
  • Received:2002-02-07 Revised:2002-06-17 Online:2003-02-25 Published:2003-02-25

摘要: 对诱导黄瓜直接器官发生的主要因素进行了探讨。结果表明: 外植体类型、基因型及植物生长调节剂对再生频率均有显著影响。子叶节是最佳的外植体类型; ‘长春密刺’及‘龙杂黄七号’再生频率较高, 分别达到93. 2 %和92. 5 % , 前者每块外植体上的平均芽数(13. 2) 极显著高于后者(3. 1) ; 62BA 单独存在即可诱导产生丛生芽, 0. 5 mg/ mL 最佳。

关键词: 黄瓜, 高效再生体系, 直接器官发生, 芽分化

Abstract: The main factors influencing the frequency of direct organogenesis of cucumber ( Cucumis sativus L. ) were discussed. The results showed that the types of explants , genotypes and plant growth regulator had significantly effects on regenerating frequency. The optimum explant is cotyledonary nodes. Genotypes ‘Changchun mici’and ‘Longzahuang 7’had higher frequency of regeneration which were 93. 2 % and 92. 1 % respectively. But the former differentiated much more buds (13. 2 per explant) than that of the latter (3. 1 per explant) . 6-BA can effectively induced buds and the optimum concentration is 0. 5 mg/ L.

Key words: Cucumber, High frequency regenerated system, Direct organogenesis, Differentiation rate of buds

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