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园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 227-232.

• 蔬菜 • 上一篇    下一篇

病毒诱导的基因沉默在番茄组织中的特异性分析

杨迎伍1;李正国1*;Mondher Bouzayen2

  

  1. 1重庆大学生物工程学院基因工程研究中心,重庆市功能基因及调控技术重点实验室,重庆 400044;2 UMR990 INRA/INP-ENSA 果实基因组及生物技术实验室,法国图卢兹 31326)
  • 收稿日期:2007-07-10 修回日期:2007-11-01 出版日期:2008-02-25 发布日期:2008-02-25
  • 通讯作者: 李正国

Tissue Specificity Analysis of Virus-induced Gene Silencing in Tomato

YANG Ying-wu1, LI Zheng-guo1*,and BOUZAYEN Mondher2

  

  1. (1 Key Laboratory of Functional Gene and Regulation Technologies of Chongqing, Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Chongqing 400044; 2 UMR990 INRA/INP-ENSA Toulouse Genomique et Biotechnologie des Fruits, Av de l'Agrobiopole, BP 32607, 31326 Castanet-Tolosan cedex, France)
  • Received:2007-07-10 Revised:2007-11-01 Online:2008-02-25 Published:2008-02-25
  • Contact: LI Zheng-guo

摘要:

病毒诱导的基因沉默(VIGS)是植物基因功能研究的新技术。以组成型表达gusA基因的转基因番茄为材料,通过VIGS技术沉默gusA基因,对VIGS在番茄中的组织特异性进行了初步分析。结果表明,VIGS技术能有效地诱导gusA基因在番茄植株的叶片、花、果实、侧枝、主茎及茎分枝点等部位和组织中沉默,证明VIGS在番茄中具有较强的系统性沉默特征。

关键词: 番茄, 病毒诱导的基因沉默(VIGS), 基因功能, 组织特异性

Abstract:

Virus-induced gene silencing (VIGS) is a recently developed technique for gene function studies in plants. In this study, the gusA transgenic tomato plant which constitutively expressed gusA gene was used as material. The gusA gene was silenced by VIGS for the preliminary tissue specificity analysis of VIGS in tomato. The results showed that gusA could be silenced in leafs, flowers, fruits, branches, stems and the points between branch and stem of tomato by virus-induced gene silencing. So VIGS as a powerful tool for the study of gene function could induce target gene systematic silencing in tomato.

Key words: Tomato, Virus-induced gene silencing (VIGS), Gene function, Tissue specificity

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