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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 217-220.

• 研究简报 • 上一篇    下一篇

与大白菜橘红心基因紧密连锁的SCAR标记

王国臣1,2; 张凤兰1*; 余阳俊1; 张德双1; 赵岫云1   

  1. (1国家蔬菜工程技术研究中心, 北京100097; 2首都师范大学生命科学学院, 北京100037)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)

WANG Guo-chen1,2; ZHANG Feng-lan1*
;YU Yang-jun1; ZHANG De-shuang1 ; ZHAO Xiu-yun1
  

  1. ( 1 National Engineering Research Center for Vegetables, Beijing 100097, China; 2 College of Life Science, CapitalNormal University, Beijing 100037, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 利用游离小孢子培养获得的DH系群体和BSA法对与大白菜橘红心基因紧密连锁的分子标记
进行了研究, 筛选到与橘红心球色基因or连锁的RAPD标记S1338656和AFLP标记P67M54172 , 其遗传距离
分别为8 cM和13 cM, 并将其成功转化成SCAR标记SCOR204和SCOR127。对标记的通用性在110个大白菜
自交不亲和系育种材料中进行了验证, 与田间鉴定结果的吻合率分别为90%和89.1%。SCAR标记的获得
为大白菜橘红心性状的分子标记辅助选择与基因的图位克隆奠定了基础。

关键词: 大白菜, 球叶色, BSA, SCAR, 标记辅助选择

Abstract: Bulked segregant analysis was emp loyed to identify molecular markers linked to orange head
leaf gene ( or) trait based on RAPD and AFLP in Chinese cabbage with a doubled hap loid (DH) population
derived from the F1 between the two parents 91-112 (white head leaf) and T12-19 ( orange head leaf) via
microspore culture. One RAPD marker S1338656 and one AFLP marker P67M54172 were identified to be linked
to or gene with the distance of 8 cM and 13 cM respectively. These two markerswere successfully transformed
into SCAR markers SCOR204 and SCOR127. The accuracy of 90% and 89.1% were obtained when 110 differ
ent incompatibility breeding lines of Chinese cabbage were used to be investigated with two SCAR markers, in
dicating that these two SCAR makers could be used in marker2assisted2selection in orange head leaf breeding
p rograms and positional cloning of or gene in Chinese cabbage.

Key words: Chinese cabbage, Brassica rapa L. ssp. pekinensis, Orange head leaf gene (or), BSA, SCAR, Marker-assisted selection