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园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 115-116.

• 研究报告 • 上一篇    下一篇

醉蝶花离体培养植株再生研究

王健美;高辉;刘志斌;吴俊;李旭锋*   

  1. (四川大学生命科学学院, 成都610064)
  • 收稿日期:2003-04-14 修回日期:2003-07-01 出版日期:2004-02-25 发布日期:2004-02-25
  • 通讯作者: 李旭锋

Studies on Plant Regeneration of Cleome spinosa Jacq. in Vitro

Wang Jianmei ;Gao Hui;Liu Zhibin;Wu Jun;Li Xufeng*   

  1. ( College of life science , Sichuan University , Chengdu 610064 , China)
  • Received:2003-04-14 Revised:2003-07-01 Online:2004-02-25 Published:2004-02-25
  • Contact: Li Xufeng

摘要: 诱导醉蝶花下胚轴和子叶形成愈伤组织, 以MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L培养基中子叶的出愈率最高, 达到100 % , 下胚轴出愈率最高只有93 %。MS + 6-BA 2 mg/L + NAA 0.05 mg/L最适合于芽的诱导, 诱导率达到100 %。再生苗在MS + NAA 0.1 mg/L 培养基中生根完成植株再生。带单个侧芽的茎作为外植体接种在MS + 6-BA 1 mg/L + NAA 0.05 mg/L 培养基中, 一个月以后形成无数丛生芽。

关键词: 醉蝶花, 离体培养, 植株再生

Abstract: Taking MS as basic media , adopting 6-BA , NAA , KT of different concentration compoundings to induce callus from cotyledons and hypocotyls of Cleome spinosa Jacq. , the result showed that the mediumof MS +6-BA 0.5 mg/ L + NAA 0.5 mg/ L + KT 0.5 mg/ L was the best to induce callus from cotyledons with a rate of 100 %frequency , and the highest rate of hypocotyl callus inducement was 93 %. And the differentiation medium MS + 6-BA 2 mg/ L + NAA 0.05 mg/ L was the best to induce buds and had a 100 % plant regeneration. And it was for rooting media , MS + NAA 0.1 mg/ L ,to produce roots from seedings. Taking the stems with single bud as explants on MS + 6-BA 1 mg/ L + NAA 0.05 mg/ L , it was found that there are lots of buds after a month.

Key words: Cleome spinosa Jacq., Culture in vitro, Plant regeneration

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