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园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 103-105.

• 研究报告 • 上一篇    下一篇

山东省黄瓜地方品种资源亲缘关系的AFLP 分析

王志峰1;孙日飞2;孙小镭1;顾兴芳2;曹齐卫1;曲士松1   

  1. (1 山东省农业科学院蔬菜研究所, 济南250100 ; 2 中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2003-01-10 修回日期:2003-09-25 出版日期:2004-02-25 发布日期:2004-02-25

Studies on Genetic Relationship among Local Cucumber Germplasm of Shandong Province by AFLP Analysis

Wang Zhifeng1; Sun Rifei2;Sun Xiaolei1;Gu Xingfang2;Cao Qiwei1; Qu Shisong1   

  1. (1 Institute of Vegetables of Shandong Academy of Agricultural Sciences, Ji'nan 250100 , China ; 2 Institute of Vegetables and Flowers ,Chinese Academy of Agricultural Sciences , Beijing 100081 , China)
  • Received:2003-01-10 Revised:2003-09-25 Online:2004-02-25 Published:2004-02-25

摘要: 利用AFLP 技术对包括80 份山东黄瓜地方品种和24 份其他地区品种的遗传亲缘关系进行了研
究。用11 对引物进行选择性扩增, 21%的扩增条带表现多态性。聚类分析结果显示: 山东黄瓜地方品种与日本品种和欧美品种分属不同类群或亚类群, 山东地方品种分为8 组, 各组内生态类型基本一致。试验得到不同生态型黄瓜的特征差异带和它们之间的遗传距离。

关键词: 黄瓜, 种质资源, AFLP, 亲缘关系, 聚类分析

Abstract: AFLP analysis was applied to study the genetic relationship among 80 local cucumber ( Cucumis sativus L. ) cultivars from Shandong province and 24 cultivars collected from other regions. 11 pair of primers were screened from 128 pair of primers and generated 559 bands , 21 % of which were polymorphic. Cluster analysis showed that the accessions cultivated in Shandong , Jinan , Europe and America were divided into different groups or
sub-groups. While local cultivars of Shandong province were divided into 8 sub-groups. In addition , specific markers of four ecotypes and the genetic distance between them were also obtained.

Key words: Cucumber, Germplasm, AFLP, Genetic relations, Cluster analysis

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