大百合子房的离体培养

园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 197-200.

大百合子房的离体培养

李守丽^1,2 ;石　雷^1*
; 张金政¹ ; 庄　平³

(¹ 中国科学院植物研究所, 北京100093; ² 中国科学院研究生院, 北京100039; 3 中国科学院华西亚高山植物园,四川都江堰611830)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

In Vitro Ovary Culture of Cardiocrinum giganteum

LI Shou-li^1,2 ; SHI Lei^1*
; ZHANG J in-zheng¹ ; ZHUANG Ping³

(¹ Institute of B otany, the Chinese Academy of Sciences, Beijng 100093, China; ² Graduate School of the Chinese Academy of Sciences, Beijing 100039, China; ³ West China Subalpine Botanical Garden, Institute of Botany, the Chinese Academy of Sciences,Dujiangyan, Sichuan 611830, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： 以大百合的子房为外植体, 研究了小鳞茎诱导及植株再生的方法。结果表明: BA和KT是影
响大百合子房分化途径的关键因素, 其浓度分别为0.1～1.0 mg/L、2.0～4.0 mg/L和高于4.0 mg/L 时,
外植体分别分化为愈伤组织、芽和叶。外植体分化的基本培养基以N6、B5为佳。愈伤组织诱导小鳞茎的
最佳培养基为MS + 0.1～0.5 mg/L NAA + 2.5 mg/L BA + 2.5 mg/L KT + 10%蔗糖+ 0.7%琼脂。在1/2MS
+ 3%蔗糖+ 0.7%琼脂+ 1%活性炭的生根培养基上, 生根率为100%。炼苗一周后移栽, 长势良好。

关键词: 大百合, 组织培养, 子房

Abstract: An experiment was carried out on the induction of bulbulets and the plant regeneration by
using the ovaries of Cardiocrinum giganteum as exp lants. The results indicated that BA and KT were the main
factors in ovary explant differentiation. The explants differentiated into calluses when the concentration of BA
and KT was between 0.1 and 1.0 mg/L and budswere induced when the concentration was bewteen 2.0 and
4.0 mg/L, and when higher than 4.0 mg/L, leaves formed. The best basic media for the dedifferentiation
were N6 and B5. The bestmedium for bulbulet induction from calluswasMS + 0.1 - 0.5 mg/L NAA + 2.5
mg/L BA + KT 2.5 mg/L + 10% sucrose + 0.7% agar. The ratio of root induction from calluswas 100% on
the medium of 1/2MS + 3% sucrose + 0.7% agar + 1% active carbon. After acclimatization for one week,
the bulblets were transp lanted into greenhouse and all grew well.

Key words: Cardiocrinum giganteum, Tissue culture, Ovary

李守丽;;石　雷;张金政;庄　平. 大百合子房的离体培养[J]. 园艺学报, 2007, 34(1): 197-200.

LI Shou-li;;SHI Lei;ZHANG J in-zheng;ZHUANG Ping. In Vitro Ovary Culture of Cardiocrinum giganteum[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 197-200.

[1]	吴致君, 李伟, 王兴华, 张成, 蒋勋, 黎盛, 罗理勇, 孙康, 曾亮. 基于SLAF-seq的茶组子房室数不稳定居群种质亲缘关系分析[J]. 园艺学报, 2022, 49(11): 2455-2470.
[2]	张文心, 曹雪莹, 周锦, 周世明, 张别鱼, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵3号’[J]. 园艺学报, 2021, 48(S2): 2909-2910.
[3]	曹雪莹, 张文心, 周锦, 周世明, 张别鱼, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵4号’[J]. 园艺学报, 2021, 48(S2): 2911-2912.
[4]	官敏华, 曹雪莹, 张文心, 叶宇壮, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵5号’[J]. 园艺学报, 2021, 48(S2): 2913-2914.
[5]	邓怿, 张文心, 曹雪莹, 房林, 李琳, 吴坤林, 刘海涛, 曾宋君, . 朱顶红新品种‘双艳’[J]. 园艺学报, 2021, 48(S2): 2915-2916.
[6]	周霞 1，张璐 1，周俊国 2，邓英 3，王怡 1，娄群峰 1，陈劲枫 1,*. 黄瓜未授粉子房离体培养获得胚囊再生植株[J]. 园艺学报, 2020, 47(3): 455-466.
[7]	邓莎1,2，曹雪莹3,4，郑枫1，张文心3,4，周世明3,4，张别鱼3,4，张金龙3,4，房林1，李琳1，吴坤林1，曾宋君1,5,*. 朱顶红新品种‘圣茵1号’[J]. 园艺学报, 2019, 46(S2): 2865-2866.
[8]	邓莎1,2，张文心3,4，郑枫1，曹雪莹3,4，周世明3,4，张别鱼3,4，张金龙3,4，房林1，李琳1，吴坤林1，曾宋君1,5,*. 朱顶红新品种‘圣茵2号’[J]. 园艺学报, 2019, 46(S2): 2867-2868.
[9]	高洁，张萍，薛璟祺，薛玉前，王顺利，张秀新. 酚类物质及其对木本植物组织培养褐变影响的研究进展[J]. 园艺学报, 2019, 46(9): 1645-1654.
[10]	韩毅科，杜胜利，魏爱民，陈正武，刘楠，张海鹏，李波. 露地黄瓜新品种‘津优409’[J]. 园艺学报, 2016, 43(8): 1627-1628.
[11]	许志茹，陈智华，姜艳东，侯杰，佟玲，李玉花*. 露地菊离体再生体系建立及BrDFR基因遗传转化[J]. 园艺学报, 2013, 40(8): 1517-1526.
[12]	刘颖颖，刘世琦*，薛小艳，刘景凯，陈祥伟，冯磊. 大蒜未受精子房离体诱导单倍体的研究[J]. 园艺学报, 2013, 40(6): 1178-.
[13]	韩晓勇，闫瑞霞，殷剑美*，张培通，郭文琦，李春宏. ‘台州紫山药’试管薯诱导体系研究[J]. 园艺学报, 2013, 40(10): 1999-2005.
[14]	张倩, 王华芳. 牡丹试管苗生根与移栽技术研究进展[J]. 园艺学报, 2012, 39(9): 1819-1828.
[15]	李杨, 余蓉培, 李慧, 李东, 石雷. 观赏蕨类植物组织培养研究进展[J]. 园艺学报, 2012, 39(9): 1839-1848.