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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 183-188.

• 观赏植物 • 上一篇    下一篇

一氧化氮对瓶插月季呼吸作用及相关酶活性的影响

张少颖;饶景萍*; 任小林   

  1. (西北农林科技大学园艺学院, 陕西杨凌712100)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Effects of Nitric Ox ide on Respiration and Enzymatic Activity of Cut RoseDuring Va se

ZHANG Shao-ying; RAO Jing-ping*
; REN Xiao-lin
  

  1. (College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 以硝普钠( SNP) 为一氧化氮供体, 研究了NO及其清除剂PTIO (2-phenyl-4, 4, 5, 5-tetra
methylimidazoline-1-oxyl-3-oxide) 处理对切花月季瓶插过程中生理变化及呼吸作用相关酶活性变化的影响。
结果表明: 0.1 mmol·L-1 SNP释放的NO可以降低月季切花的萎蔫率, 延长瓶插寿命, 增加花枝鲜样质
量, 抑制COX (细胞色素氧化酶) 、AO (抗坏血酸氧化酶) 、PPO (多酚氧化酶) 活性, 抑制月季呼吸强
度和乙烯释放, 延缓二者跃变高峰的出现, 延缓MDA含量和膜相对透性的升高, PTIO的处理提高了上述
SNP处理下COX、AO、PPO的活性, 提高了呼吸速率, 促进MDA含量和膜相对透性的上升, 但抑制了乙
烯的释放, 初步说明NO可能参与衰老过程中呼吸作用的末端氧化酶的活性调节。

关键词: 切花月季, 呼吸作用, 末端氧化酶, 衰老

Abstract: Using sodium nitrop russide ( SNP) as a nitric oxide (NO) donor and its scavenger PTIO (2-phenyl-4, 4, 5, 5-tetra
methylimidazoline-1-oxyl-3-oxide) , the effects of nitric oxide treatment on respira
tion, ethylene p roduction, relative membrane permeability, MDA content and activities of COX ( cytochrome
oxidase) , AO ( ascorbic acid oxidase) and PPO (polyphenol oxidase) of cut rose were studied. The results
showed that NO resulting from 0.1 mmol·L-1 SNP aqueous solution decreased wilt rate, increased fresh
mass, p rolonged vase life, inhibited COX, AO and PPO activities, decreased the resp iration rate and ethylene
p roduction, delayed the resp iration and ethylene peaks, and slowed down the increase ofMDA content and the
relative membrane permeability. The treatment of PTIO stimulated COX, AO and PPO activities, increased
the resp iration rate, accelerated the increase ofMDA content and relative membrane permeability under SNP
treatment, but inhibited the ethylene p roduction. Those results indicated that NO might participate in regula
tion of terminal oxidases in resp iration.

Key words: Rose, Rosa hybrida, Respiration, Terminal oxidase, Senescence