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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 143-146.

• 蔬菜 • 上一篇    下一篇

辣椒C基因全长序列比较分析

王 岩1,2 ;张宝玺1 ; 张延国1 ; 张国裕1 ; 王立浩1 ; 徐有明2 ;王晓武1*   

  1. (1 中国农业科学院蔬菜花卉研究所, 北京100081; 2 华中农业大学园艺林学学院, 武汉470070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Comparison and Analysis of C Gene from Different Pepper Accessions

WANG Yan1,2 ; ZHANG Bao-xi1 ; ZHANG Yan-guo1 ; ZHANG Guo-yu1; WANGLi-hao1 ; XU You-ming2 ;
WANG Xiao-wu1*
  

  1. (1 Institute of Vegetables and Flowers, Chinese Academ y of Agricultural Sciences, Beijing 100081, China; 2 College of Horticultureand Forestry, Huazhong Agricultural University, Wuhan 430070, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 辣/甜椒C基因测序比对后证实了甜椒C基因5′端约689 bp的缺失可能是造成其辣味缺失的
原因。牛角形甜椒‘0538’的C基因不存在这一缺失, 却在编码区第40位碱基处有一个G到A的突变,
可能导致信号肽内第14位疏水性天冬氨酸变为亲水性天冬酰胺, 不能完成辣椒素的合成, 最终辣味缺失。
辣/甜椒品种共有长片段Catf2基因, 与C基因在核苷酸序列上有88.9%的相似性。

关键词: 辣椒, 辣椒素合成酶, 序列分析, 信号肽

Abstract: Deletion of 689 bp in 5′- terminal of the C gene locus caused an absence of pungency in sweet
pepper after sequencing and alignment. A C gene fragment from non-pungent oxhorn pepper 0538 was amp li
fied, which does not have the deletion, but a mutation from G to A at 40 bp in the coding region. Thismuta
tion led to the change of an amino acid from hydrophobic aspartic acid to hydrophilic asparaginate in signal
pep tide, which might cause the loss of the function of the C gene and finally the pungency. Using the same
primer pair, Catf2 gene from all used accessions was amp lified, which shares high similarity about 88.9%
with the C gene.

Key words: Pepper, Capsicum annuum L., Capsaicinoid synthetase, Sequence analysis, Signal peptide