芸芥体细胞胚胎发生的组织细胞学研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 131-134.

芸芥体细胞胚胎发生的组织细胞学研究

张　涛^1,2

(¹ 重庆师范大学生命科学学院, 重庆400047; ² 甘肃省农业科学院, 兰州730070)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Histological Stud ies of Somatic Embryogenesis in Eruca sativa Mill.

ZHANG Tao^1,2

(¹ College of Life Sciences, Chongqing Normal University, Chongqing 400047, China; ²Gansu Academy of Agricultural Sciences,Lanzhou 730070, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： 以芸芥子叶为外植体, 在MS + 2, 4-D 1.0 mg·L^-1培养基上培养2周后可诱导体细胞胚胎的
发生。胚性愈伤组织在MS + 2, 4-D 0.2 mg·L^-1培养基上大量增殖, 体细胞胚胎在N₆培养基上成熟后, 可
在附加0.1 mg·L^-1 IBA的1/2MS培养基上生长。切片观察表明: 芸芥体细胞胚胎为单细胞发生方式, 与合
子胚形成过程相似。芸芥胚性细胞具有细胞小、胞质浓、核大、核仁明显, 排列紧密的特点, 首先由单个
细胞分裂形成2 - 细胞原胚, 2 - 细胞原胚分裂成3 - 细胞原胚或4 - 细胞原胚, 3 - 细胞原胚或4 - 细胞原
胚继续分裂形成多细胞原胚, 进一步发育成一个成熟的体细胞胚胎。

关键词: 芸芥, 体细胞胚胎, 组织细胞学

Abstract: Somatic embryos were induced from cotyledon explants cultured on Murashige and Skoog
(MS) medium supplemented with 1.0 mg·L^-1 2, 4-D after two weeks of culture. Embryogenic callus prolif
erated p rofusely on MS medium containing 0.2 mg·L^-1 2, 4-D. Somatic embryosmatured on N₆ medium and
developed into p lantlets by culturing the mature somatic embryos on 1/2 MS medium containing 0.1 mg·L^-1
indole-3-butyric acid. Section and microscop ic observation of different developmental stages revealed that so
matic embryogenesis was similar to that of the zygotic embryo. It originated from a single embryogenic cell,
which had bigger nuclei, thick cytop lasm, clear nucleoli and denser order. The embryogenic cell first divided
into two-celled stage proembryo, then three- or four-celled stage proembryo. Three- or four-celled stage pro
embryo developed into multicellular proembryo, then developed into mature somatic embryo.

Key words: Eruca sativa, Somatic embryos, Histological

张　涛;. 芸芥体细胞胚胎发生的组织细胞学研究[J]. 园艺学报, 2007, 34(1): 131-134.

ZHANG Tao;. Histological Stud ies of Somatic Embryogenesis in Eruca sativa Mill.[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 131-134.

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