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园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 215-216.

• 研究报告 • 上一篇    下一篇

柑橘体细胞杂种超低温保存后的植株再生

王子成;邓秀新
  

  1. (华中农业大学作物遗传改良国家重点实验室,武汉430070)
  • 收稿日期:2003-05-27 修回日期:2003-07-28 出版日期:2004-04-25 发布日期:2004-04-25

Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus

Wang Zicheng;Deng Xiuxin
  

  1. (The National Key Laboratory ofCrop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China)
  • Received:2003-05-27 Revised:2003-07-28 Online:2004-04-25 Published:2004-04-25

摘要: 对9年生印度酸橘(Citrus reticaulata)和飞龙枳(Poncirus trifoliata)的体细胞杂种的茎段进行离体培养得到再生植株,用改良的玻璃化法对再生植株茎尖进行超低温保存后获得92%的再生率。这是柑橘体细胞杂种茎尖超低温保存的首例报道。

关键词: 柑橘, 体细胞杂种, 离体培养, 超低温保存

Abstract: The aUotetraploid citrus somatic hybrid is a new kind of germplasm created via protoplast fusion. We have succeeded in culturing the stems of 9 year-old tree of the somatic hybrid of Cleopatra Mandarin (Citrus reticaulata)and Flying Dragon(Poncirus trifoliata)in vitro,later cryopreserved its shoot tips from in vitro and got regeneration rate by 92% of the cryopreserved shoot-tips.This is the first report on the cryopreservation of citrus somatic hybrid shoot-tips.

Key words: Citrus, Somatic hybrid, Culture in vitro, Cryopreservation

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