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园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 199-204.

• 研究论文 • 上一篇    下一篇

辣椒花药培养中若干影响因素的研究

王立浩;张宝玺;郭家珍;杨桂梅;堵玫珍
  

  1. (中国农业科学院蔬菜花卉研究所,北京100081)
  • 收稿日期:2003-10-09 修回日期:2004-02-24 出版日期:2004-04-25 发布日期:2004-04-25
  • 通讯作者: 张宝玺

Studies of Effects of Several Factors on Anther Culture of Capsicum annuum L.

Wang Lihao;Zhang Baoxi ;Guo Jiazhen;Yang Guimei; Du Meizhen
  

  1. (Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Received:2003-10-09 Revised:2004-02-24 Online:2004-04-25 Published:2004-04-25
  • Contact: Zhang Baoxi

摘要: 以4个辣椒品种(组合)为试材,通过正交试验,按不同取蕾时期,在培养基中添加不同浓度的硝酸银,维生素C、植物生长调节剂、氮源、碳源、核酸、活性炭等研究对辣椒花药培养中组织褐化、愈伤组织发生率、胚状体的发生率的影响。结果表明:硝酸银、维生素c能有效地减轻组织褐化;对于辣椒花药培养愈伤组织生成率影响较大的前4位依次是植物生长调节剂、活性炭、硝酸银、维生素C;对于胚状体发生率影响较大的前4位依次是维生素C、取蕾时期、碳源、硝酸银。

关键词: 辣椒, 花药培养, 愈伤组织, 胚状体发生, 褐化, 培养基

Abstract: Four genotypes of pepper were used to study the effects of AgNO3,Vitamin C,carbon source,nitrogen source,harvest time,plant growth regulator on the anther culture of Capsicum annuum L. through orthogonal tests.As the results,AgNO3,Vitamin C can reduce the browning of the tissues;Plant growth regulator,active carbon,AgNO3,Vitamin C have a higher effect on calli yields;Vitamin C,harvest time,carborn source,AgNO3 have a higher effect on embryogenesis.A new modified culture medium was concluded.

Key words: Capsicum annuum L., Anther culture, Embryogenesis, Callus, Browning, Medium

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