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园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1063-1066.

• 研究报告 • 上一篇    下一篇

梨矮化中间砧S2 、S5 和PDR54的离体培养研究

罗娅;汤浩茹;李秀梅;王小蓉   

  1. (四川农业大学林学园艺学院, 四川雅安 625014)
  • 收稿日期:2005-09-01 修回日期:2006-03-09 出版日期:2006-10-25 发布日期:2006-10-25

Study on Tissue Culture in Vitro of Pear Dwarf ing Interstocks S2 , S5 andPDR54

Luo Ya;Tang Haoru;Li Xiumei;Wang Xiaorong   

  1. ( Forestry and Horticultural College, Sichuan Agricultural University, Ya'an, Sichuan 625014, China)
  • Received:2005-09-01 Revised:2006-03-09 Online:2006-10-25 Published:2006-10-25

摘要:

以梨矮化中间砧S2、S5和PDR54试管苗为试材, 探讨了培养基和植物生长调节剂对梨矮化中间砧试管苗增殖和生根的效应。其结果表明: MS培养基较QL、AS和WPM培养基更有利于中间砧茎的增殖, 2~3 mg·L - 1 6-BA、0.1~0.2 mg·L - 1 IBA和1~2 mg·L - 1 GA3组合能有效促进S2、S5和PDR54试管苗的增殖, 其增殖倍数达3.71~5.83。综合各品种增殖倍数、茎高度以及茎质量等指标表明, MS + 3.0 mg·L -1 6-BA + 0.2 mg·L-1 IBA + 2.0 mg·L-1 GA3是S2的最佳增殖培养基; MS + 2.0 mg·L-1 6-BA + 0.1 mg·L-1 IBA + 1.0 mg·L-1 GA3是S5和PDR54增殖的最佳培养基。同时, S2、S5和PDR54增殖的试管苗在不同的生根条件下分别获得了67%、50%和86%的生根率。

关键词: 梨, 矮化中间砧, 快繁

Abstract: The influencing factors of multiplication coefficient, regeneration quality and rooting for adventitious shoots of pear dwarfing interstocks S2 , S5 and PDR54 were studied. The results showed that the regeneration ability of adventitious shoots was influenced by genotypes, media and plant growth regulators. MS
medium was more suitable for the proliferation of S2 , S5 and PDR54 than QL, WPM and AS media. The middle concentrations of 6-BA (2 - 3 mg·L - 1 ) in combination with lower concentrations of IBA (0.1 - 0.2 mg·L - 1 ) and GA3 (1 - 2 mg·L-1 ) could improve the proliferation of all the three interstocks, with proliferous index of shoots from 3.71 to 5.83. The best medium for S2 was MS + 3.0 mg·L - 1 6-BA + 0.2mg·L - 1IBA + 2.0 mg·L - 1 GA3 , while MS + 2.0 mg·L - 1 6-BA + 0.1 mg·L - 1 IBA + 1.0 mg·L - 1 GA3 was the optimal for S5 and PDR54 proliferation in comparison of the comprehensive index of shoot quality, numbers and lengths. The regenerated shoots were rooted with a rooting percentage of 67% , 50% and 86% respectively for S2 , S5 and PDR54.

Key words: Pear, Dwarfing interstock, Rapid propagation