关键词: 苹果, 花青素, 5 - 氨基乙酰丙酸(ALA), 金雀异黄素(GNT), PAL活性

Abstract: Two plant endogenous substances, 5-aminolevulinic acid (ALA ) and genistein ( GNT) ,were proposed to p romote anthocyanin accumulation of apple fruits in the paper. When 300 mg·L ^{- 1} ALA was app lied to ‘Fuji’apples 43 days before mature harvest, the rate of anthocyanin accumulation and the final content were both almost doubled compared with that of the control. When ALA was applied to the detached fruit discs followed by 48 hour continuous white light irradiation about 160 μmol·m^-2 ·s^-1 , the anthocyanin content rose about 2 times as high as that of the light control, and the latter 2 times as high as that of the dark control. A significant promotion on anthocyanin accumulation was firstly observed in treatment with GNT, where the anthocyanin content in the treated ‘Fuji’ app le discs was about 10 times as high as that of the dark control, about 2 times as effective as ALA treatment. Furthermore, 80 μmol·L ^{- 1} GNT treatment could significantly promote anthocyanin accumulation in‘Golden Delicious’ app le discs, which has seldom been reported before. Additionally, the results showed that promotion ofALA and GNT on anthocyanin accumulation under continuous light was related with induction of phenylalanine ammonialyase ( PAL) activity, because it increased temporarily afterALA and GNT treatments. ALA and GNT are hopeful to be potentially used in fruit production.

Key words: Apple, Anthocyanin, 5-aminolevulinic acid (ALA), Genistein (GNT), PAL activity