大白菜与诸葛菜属间杂交及GISH鉴定

园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1473-1478.

大白菜与诸葛菜属间杂交及GISH鉴定

乔海云;李菲;张淑江;章时蕃;孙日飞*

(中国农业科学院蔬菜花卉研究所, 北京100081)

收稿日期:2009-04-27 修回日期:2009-06-24 出版日期:2009-10-25 发布日期:2009-10-25
通讯作者: 孙日飞

Intergeneric Hybridization of Brassica campestris L. ssp. pekinensis (Lour. )Olsson and Orychophragmus violaceus and GISH Identification

QIAO Hai-yun;Li Fei;ZHANG Shu-jiang;ZHANG Shi-fan;SUN Ri-fei*

(Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2009-04-27 Revised:2009-06-24 Online:2009-10-25 Published:2009-10-25
Contact: SUN Ri-fei

摘要/Abstract

摘要： 以不同类型的大白菜与诸葛菜进行属间正反杂交, 运用蕾期重复授粉及胚挽救技术, 在大白菜为母本的组合中获得了31株幼苗, GISH鉴定表明有16株具有预期的染色体数, 2n = 22, 为真杂种; 在诸葛菜为母本的组合中没有得到杂种植株。田间调查显示, 杂种一代植株综合性状介于双亲之间, 同时花粉败育。

关键词: 大白菜, 诸葛菜, 属间杂交, GISH

Abstract: The intergeneric hybridizations between different Brassica campestris L. ssp. pekinensis(Lour. ) Olsson and Orychophragmus violaceus were carried out. By successive bud pollination and ovule culture, B. campestris L. ssp. pekinensis and O. violaceus were crossed reciprocally. When B. campestris L. ssp. pekinensis was used asmother plants, thirty-one plants were obtained, while O. violaceus was used asmother plants, no plants were rescued. Sixteen of the thirty-one p lants showed characteristics of both parents. GISH identification revealed that sixteen of the thirty-one hybrid plants were real hybrids because they had the anticipative chromosomes (2n = 22). The investigation in the field showed that many characteristics in between both parents and the pollen fertility of hybrid plants ( F₁ ) was aborted.

Key words: Brassica campestris L. ssp. pekinensis (Lour. ) Olsson, Orychophragmus violaceus (L. )O. E. Schulz, intergeneric cross, GISH

中图分类号:

S 634.1

乔海云;李菲;张淑江;章时蕃;孙日飞. 大白菜与诸葛菜属间杂交及GISH鉴定[J]. 园艺学报, 2009, 36(10): 1473-1478.

QIAO Hai-yun;Li Fei;ZHANG Shu-jiang;ZHANG Shi-fan;SUN Ri-fei. Intergeneric Hybridization of Brassica campestris L. ssp. pekinensis (Lour. )Olsson and Orychophragmus violaceus and GISH Identification[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1473-1478.

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